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SCHIFF’s Reagent

Schiff's reagent is a staining solution used in histology and cytology. It consists mainly of a solution of basic fuchsin reduced with sodium metabisulfite or sodium disulfite. The reagent is used primarily in what is known as Schiff fuchsin staining, which is based on the ability of aldehydes to react with reduced dyes to produce a specific stain.

In application, Schiff's reagent is commonly used to visualize periodic acid-Schiff (PAS)-reactive structures in tissues. PAS staining is an important method for detecting polysaccharides, glycoproteins, and other structures containing aldehydes, such as glycogen, mucus, and basement membranes.

After pretreating tissue sections with periodic acid, which generates aldehyde groups, Schiff's reagent is applied. The aldehyde groups react with the reduced dye in the reagent, producing an intense magenta stain. This staining allows the identification and localization of PAS-reactive structures in the tissues examined.

Article no.: 11686

Detection of aldehyde groups

  •   Container size and order number Net price Amount
    SCHIFF’s Reagent - 100 ml
    Order number: 11686.00100
    19,40 €
    SCHIFF’s Reagent - 250 ml
    Order number: 11686.00250
    23,15 €
    SCHIFF’s Reagent - 500 ml
    Order number: 11686.00500
    32,90 €
    SCHIFF’s Reagent - 1.000 ml
    Order number: 11686.01000
    59,49 €
    SCHIFF’s Reagent - 2.500 ml
    Order number: 11686.02500
    108,94 €
    SCHIFF’s Reagent - 60.000 ml
    Order number: 11686.60000
    814,54 €

Important Information

UN-Nummer: 0000

Warning labels:

Lagerung: 4 … 20 °C

Haltbarkeit: 9 Monate

safety data sheet


product information

Relevant Incredients:
• Hydrochloric Acid 37%
• Pararosaniline (C.I.: 42500)
• Sodium metabisulfite
• Charcoal, med.

instruction manual

Technical informations

specifications

Instructions / Protocol / Recommendations

Verwendung:

Schiff's reagent is a staining solution used in histology and cytology to visualize periodic acid-Schiff (PAS)-reactive structures in tissues. It allows identification and localization of polysaccharides, glycoproteins, and other aldehyde-containing structures such as glycogen, mucus, and basement membranes. The aldehyde groups react with the reduced dye in the reagent, producing an intense magenta color. It is used primarily in Schiff fuchsin staining and plays an important role in in vitro diagnostics.

Prinzip:

SCHIFF's reagent is an important staining solution in in vitro diagnostics, used because of its specific binding to tissue or cell structures. The principle of operation is based on the reaction of pararosaniline, a basic dye, with aldehyde groups present in the cell structures or tissues under investigation. Sodium disulfite is used as a reducing agent to convert the pararosaniline to a colorless form (leucoform). The hydrochloric acid provides an acidic environment that facilitates the reaction. Upon contact with aldehydes in the sample, the color of the pararosaniline is restored, resulting in a visible coloration. The medicinal charcoal removes unbound dye residues to ensure clear staining.

Verfahren:

SCHIFF's reagent is an important staining solution in in vitro diagnostics, used because of its specific binding to tissue or cell structures. The principle of operation is based on the reaction of pararosaniline, a basic dye, with aldehyde groups present in the cell structures or tissues under investigation. Sodium disulfite is used as a reducing agent to convert the pararosaniline to a colorless form (leucoform). The hydrochloric acid provides an acidic environment that facilitates the reaction. Upon contact with aldehydes in the sample, the color of the pararosaniline is restored, resulting in a visible coloration. The medicinal charcoal removes unbound dye residues to ensure clear staining.

Hazard and safety instructions

Signal Word 1: Gefahr
Signal Word 2: silhouete, , ,
Warning labels:

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