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Staining kit: DNA staining according to FEULGEN

The Staining Kit: DNA Staining according to FEULGEN is an in vitro diagnostic developed to selectively detect and quantify DNA in basophilic tissue. The stain is characterized by its specific binding to deoxyribose in DNA, making it a reliable tool for DNA quantification.

The application of the kit involves acid hydrolytic pretreatment of the tissue with hydrochloric acid to release the deoxyribose for subsequent staining. In the next step, Schiff's reagent binds to the released aldehyde group of the deoxyribose to produce a red-purple stain.

The sodium disulfite serves as an antioxidant to prevent oxidation of the reagent, ensuring stability and reproducibility of the stain. Basophilic tissue is stained green, which increases contrast and allows better visualization of DNA.

The result is a precise and quantitative representation of DNA in the cell that can be analyzed by photometric methods.

Article no.: 15631

DNA staining

  •   Container size and order number Net price Amount
    Staining kit: DNA staining according to FEULGEN - 100 ml
    Order number: 15631.00100
    33,86 €
    Staining kit: DNA staining according to FEULGEN - 250 ml
    Order number: 15631.00250
    49,02 €
    Staining kit: DNA staining according to FEULGEN - 500 ml
    Order number: 15631.00500
    90,06 €
    Staining kit: DNA staining according to FEULGEN - 1.000 ml
    Order number: 15631.01000
    174,53 €
    Staining kit: DNA staining according to FEULGEN - 2.500 ml
    Order number: 15631.02500
    396,04 €

Important Information

Lagerung: siehe Einzelprodukte


product information

Components of this kit:
• Hydrochloric Acid 5.0 mol/l, Artikel-Nr.:13533
• Hydrochloric Acid 5.0 mol/l, Artikel-Nr.:13533
• SCHIFF’s Reagent, Artikel-Nr.:11686
• SCHIFF’s Reagent, Artikel-Nr.:11686
• Sodium Disulfite 10 %, Artikel-Nr.:13108

colouring protocol

Technical informations

specifications

Instructions / Protocol / Recommendations

Verwendung:

The Staining Kit: DNA Staining according to FEULGEN is an in vitro diagnostic solution specifically designed for staining DNA. The solution allows the selective detection of DNA (red-violet) in basophilic tissue (green) and is therefore ideally suited for the photometric quantification of DNA material. By using this staining method, scientists and researchers can quickly and accurately identify and analyze DNA. The combination of hydrochloric acid, Schiff's reagent and sodium disulfite provides an effective and reliable staining option for DNA in various applications and studies.

Prinzip:

The staining kit is based on the Feulgen method for DNA staining and can be used in in vitro diagnostics. The tissue or cell structures to be stained are first treated with hydrochloric acid, which causes the deoxyribonucleic acid (DNA) to break down into its individual components. Schiff's reagent is then used to stain the resulting aldehyde groups. The sodium disulfite serves as a reducing agent to remove unbound Schiff's reagent. Through this process, cell nuclei and DNA are visualized, allowing the study of cell growth, cycle and morphology.

Verfahren:

The staining kit is based on the Feulgen method for DNA staining and can be used in in vitro diagnostics. The tissue or cell structures to be stained are first treated with hydrochloric acid, which causes the deoxyribonucleic acid (DNA) to break down into its individual components. Schiff's reagent is then used to stain the resulting aldehyde groups. The sodium disulfite serves as a reducing agent to remove unbound Schiff's reagent. Through this process, cell nuclei and DNA are visualized, allowing the study of cell growth, cycle and morphology.

Hazard and safety instructions

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