Verwendung:

Schiff's reagent is a staining solution used in histology and cytology to visualize periodic acid-Schiff (PAS)-reactive structures in tissues. It allows identification and localization of polysaccharides, glycoproteins, and other aldehyde-containing structures such as glycogen, mucus, and basement membranes. The aldehyde groups react with the reduced dye in the reagent, producing an intense magenta color. It is used primarily in Schiff fuchsin staining and plays an important role in in vitro diagnostics.

Prinzip:

SCHIFF's reagent is an important staining solution in in vitro diagnostics, used because of its specific binding to tissue or cell structures. The principle of operation is based on the reaction of pararosaniline, a basic dye, with aldehyde groups present in the cell structures or tissues under investigation. Sodium disulfite is used as a reducing agent to convert the pararosaniline to a colorless form (leucoform). The hydrochloric acid provides an acidic environment that facilitates the reaction. Upon contact with aldehydes in the sample, the color of the pararosaniline is restored, resulting in a visible coloration. The medicinal charcoal removes unbound dye residues to ensure clear staining.

Verfahren:

SCHIFF's reagent is an important staining solution in in vitro diagnostics, used because of its specific binding to tissue or cell structures. The principle of operation is based on the reaction of pararosaniline, a basic dye, with aldehyde groups present in the cell structures or tissues under investigation. Sodium disulfite is used as a reducing agent to convert the pararosaniline to a colorless form (leucoform). The hydrochloric acid provides an acidic environment that facilitates the reaction. Upon contact with aldehydes in the sample, the color of the pararosaniline is restored, resulting in a visible coloration. The medicinal charcoal removes unbound dye residues to ensure clear staining.