Hematoxylin acidic according to Mayer (S) finds application in the histological examination of tissue samples, especially for the staining of basophilic structures such as cell nuclei, DNA and the rough endoplasmic reticulum. The acidic formulation reduces the staining of erythrocytes, making cell nuclei more visible.

The particular suitability of this solution lies in its use in the analysis of tissue sections where precise differentiation of cell structures is required. The chemical mode of operation is based on the formation of stable hemalaun complexes by the interaction of hematoxylin with aluminum ammonium sulfate. These complexes bind to basophilic structures and produce a characteristic blue coloration.

Compared to other hematoxylin solutions according to Mayer, the acidic variant (S) exhibits differences in composition. The content of hematoxylin and chloral hydrate is balanced in this formulation to achieve optimal staining results. Lower amounts of hematoxylin could cause less intense staining, while higher amounts of chloral hydrate could make the solution more unstable.

Overall, Mayer's hematoxylin acidic (S) provides improved staining of basophil structures in histological tissue specimens and features a balanced composition that leads to accurate and differentiated results.