Staining Kit: Silver Impregnation after GOMORI
The Gomori silver plating kit is a specialized staining kit for imaging reticulum fibers and identifying certain fungal infections in histological specimens.
The individual components of the kit have the following functions:
The Gomori silvering technique is particularly valuable in diagnostics and research because it allows highly specific visualization of structures that are difficult to detect with other staining methods. Reticulum fibers, which provide important information about tissue architecture and function, can be visualized in detail. In addition, this method offers an effective way to identify fungal infections, as the cell walls of the fungi bind the silver ions and can thus be visualized.
The individual components of the kit have the following functions:
- Potassium permanganate 0.5% and potassium metabisulfite 2% are used to pretreat tissue specimens. Oxidation followed by reduction prepares the tissue structure for subsequent silvering.
- Ammonium iron (III) sulfate 2% acts as a mordant that binds the silver nitrate to the desired cell structures.
- Silver nitrate 5% according to Fontana Masson is the primary staining solution that provides silver ions that attach to the cell structures.
- Formalin 4% is used as a fixative to stabilize the cell structures during the staining process and facilitate the binding of the silver ions.
- Gold chloride 0.1% and sodium thiosulfate 1% are used for post-staining treatment. Gold chloride tones the staining result and improves its visibility, while sodium thiosulfate stops the silvering reaction and stabilizes the staining result.
The Gomori silvering technique is particularly valuable in diagnostics and research because it allows highly specific visualization of structures that are difficult to detect with other staining methods. Reticulum fibers, which provide important information about tissue architecture and function, can be visualized in detail. In addition, this method offers an effective way to identify fungal infections, as the cell walls of the fungi bind the silver ions and can thus be visualized.
Article no.: 11104
Staining of tissue samples
Important Information
Sales restriction: no private users!
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product information
Components of this kit:
• Potassium Permanganate 0.5 %, Artikel-Nr.:11152
• Potassium Metabisulfite 2 %, Artikel-Nr.:11149
• Ammonium Iron (III) Sulfate 2 %, Artikel-Nr.:11140
• Kit: FONTANA MASSON’s Silver Nitrate 5 %, Artikel-Nr.:11377
• Formalin 4 %, carbonate buffer, pH neutral, Artikel-Nr.:10192
• Goldchloride 0.1 %, Artikel-Nr.:11134
• Sodium Thiosulfate 1 %, Artikel-Nr.:11155
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