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Staining Kit: Silver Impregnation after BIELSCHOWSKY

Staining kit: Silver impregnation according to BIELSCHOWSKY represents an outstanding instrument for histological analysis. As an in vitro diagnostic agent, it provides detailed representations of neurological structures by staining neurofibrils and reticular fibers black and collagenous fibers brown. Silver nitrate plays a central role in this staining method by interacting with the tissue structures under study and depositing a dark-appearing silver upon reduction. The use of formalin allows effective fixation of the specimens, while potassium permanganate and potassium metabisulfite provide high-contrast staining. In addition, gold chloride and sodium thiosulfate improve the staining result. Ammoniacalization of silver nitrate achieves differentiated staining. Overall, this provides improved imaging for neurological and histological analysis, which can be used to study disease states and for research.

Article no.: 16736

Dyeing reticuline fibers

  •   Container size and order number Net price Amount
    Staining Kit: Silver Impregnation after BIELSCHOWSKY - 100 ml
    Order number: 16736.00100
    134,76 €
    Staining Kit: Silver Impregnation after BIELSCHOWSKY - 250 ml
    Order number: 16736.00250
    208,79 €
    Staining Kit: Silver Impregnation after BIELSCHOWSKY - 500 ml
    Order number: 16736.00500
    423,53 €
    Staining Kit: Silver Impregnation after BIELSCHOWSKY - 1.000 ml
    Order number: 16736.01000
    815,17 €

Important Information

UN-Nummer: siehe Einzelprodukte

Warning labels:

Lagerung: siehe Einzelprodukte


product information

Components of this kit:
• Potassium Permanganate 0.25 %, Artikel-Nr.:14502
• Potassium Metabisulfite 2 %, Artikel-Nr.:11149
• Silver Nitrate 2 %, Artikel-Nr.:11183
• Formalin 4 %, carbonate buffer, pH neutral, Artikel-Nr.:10192
• Kit: Silver Nitrate 5 % with Ammonia and NaOH, Artikel-Nr.:16741
• Goldchloride 0.1 %, Artikel-Nr.:11134
• Sodium Thiosulfate 5 %, Artikel-Nr.:10288

colouring protocol

Technical informations

specifications

Instructions / Protocol / Recommendations

Verwendung:

The staining kit for silver impregnation according to Bielschowsky is used for in vitro diagnostics in the context of histological examinations. It enables the visualization of neurofibrils, reticular and collagen fibers in prepared tissue samples. The procedure stains neurofibrils and reticular fibers black, while collagenous fibers take on a brown stain. The kit composition includes solutions such as potassium permanganate, potassium metabisulfite, silver nitrate, formalin, gold chloride, and sodium thiosulfate, which are used according to the Bielschowsky method to ensure effective staining and subsequent examination of reticulin fibers in histological specimens.

Prinzip:

The silver impregnation method according to Bielschowsky is a histological staining method for visualizing nerve cells, axons and neurofibrillary structures in tissue samples. In this process, complex formation and reduction lead to the attachment of silver salts to certain cellular components. Potassium permanganate is used to pretreat and oxidize the tissues, while potassium metabisulfite and silver nitrate lead to the formation and deposition of elemental silver on the target structures. Formalin fixes the tissue samples, while ammoniacal silver nitrate solution and gold chloride stabilize and intensify the staining. Sodium thiosulfate removes excess silver and reduces non-specific staining. This method is used in in vitro diagnostics for the investigation of neurological diseases.

Verfahren:

The silver impregnation method according to Bielschowsky is a histological staining method for visualizing nerve cells, axons and neurofibrillary structures in tissue samples. In this process, complex formation and reduction lead to the attachment of silver salts to certain cellular components. Potassium permanganate is used to pretreat and oxidize the tissues, while potassium metabisulfite and silver nitrate lead to the formation and deposition of elemental silver on the target structures. Formalin fixes the tissue samples, while ammoniacal silver nitrate solution and gold chloride stabilize and intensify the staining. Sodium thiosulfate removes excess silver and reduces non-specific staining. This method is used in in vitro diagnostics for the investigation of neurological diseases.

Hazard and safety instructions

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