Staining Kit: PAS-Reaction
The kit contains 1.0% periodic acid, an oxidizing agent that is applied to oxidize carbohydrates in the tissue sample to generate aldehyde groups. These are then treated with the included Schiff reagent to produce a characteristic magenta coloration. The Schiff reagent is a fuchsin-sulfuric acid complex that reacts specifically with aldehyde groups to cause a color reaction known as Schiff's sample.
To further enhance visualization and differentiation of structures within the tissue sample, the kit contains Mayer's acidic hematoxylin. This counterstain is used to visualize cell nuclei in a blue hue.
Article no.: 12153
Differentiation of mucopolysaccharides
Important Information
Lagerung: siehe Einzelprodukte
product information
Components of this kit:
• Periodic Acid 1 %, Artikel-Nr.:11415
• SCHIFF’s Reagent, Artikel-Nr.:11686
• Hematoxylin acid according to MAYER, Artikel-Nr.:10231
Instructions / Protocol / Recommendations
Verwendung:
The PAS (Periodic Acid-Schiff) reaction is one of the most common staining in histology. The PAS staining kit is suitable for the detection of 1,2-glycogen and mucosubstances. Frozen sections, thin section preparations, and kerosene sections are used for staining. The staining solution is intended exclusively for professional use as an in vitro diagnostic in the histology laboratory.
Prinzip:
PAS staining is a histochemical staining for the detection of carbohydrate-containing components, such as glycogen. Periodic acid is used in the first step of the staining. Oxidation of 1,2-glycogen to aldehyde groups occurs. \rIn the staining step with the sulfuric acid fuchsin contained in Schiff's reagent, a color complex is formed which appears pink to purple in the histological image. In combination with the hematoxylin, the nuclei are shown in blue color.
Verfahren:
PAS staining is a histochemical staining for the detection of carbohydrate-containing components, such as glycogen. Periodic acid is used in the first step of the staining. Oxidation of 1,2-glycogen to aldehyde groups occurs. \rIn the staining step with the sulfuric acid fuchsin contained in Schiff's reagent, a color complex is formed which appears pink to purple in the histological image. In combination with the hematoxylin, the nuclei are shown in blue color.