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Staining Kit: PAS-Reaction

The PAS Reaction Staining Kit has been developed to efficiently perform staining of aldehyde groups in tissue samples. It is particularly useful for visualizing glycogen, mucopolysaccharides, proteoglycans, and other structures containing periodic acid reactive (PAS) substances that play a role in various tissues as well as pathological processes.

The kit contains 1.0% periodic acid, an oxidizing agent that is applied to oxidize carbohydrates in the tissue sample to generate aldehyde groups. These are then treated with the included Schiff reagent to produce a characteristic magenta coloration. The Schiff reagent is a fuchsin-sulfuric acid complex that reacts specifically with aldehyde groups to cause a color reaction known as Schiff's sample.

To further enhance visualization and differentiation of structures within the tissue sample, the kit contains Mayer's acidic hematoxylin. This counterstain is used to visualize cell nuclei in a blue hue.

Article no.: 12153

Differentiation of mucopolysaccharides

  •   Container size and order number Net price Amount
    Staining Kit: PAS-Reaction - 100 ml
    Order number: 12153.00100
    32,43 €
    Staining Kit: PAS-Reaction - 250 ml
    Order number: 12153.00250
    49,19 €
    Staining Kit: PAS-Reaction - 500 ml
    Order number: 12153.00500
    86,70 €
    Staining Kit: PAS-Reaction - 1.000 ml
    Order number: 12153.01000
    137,81 €
    Staining Kit: PAS-Reaction - 2.500 ml
    Order number: 12153.02500
    306,97 €

Important Information

Lagerung: siehe Einzelprodukte


product information

Components of this kit:
• Periodic Acid 1 %, Artikel-Nr.:11415
• SCHIFF’s Reagent, Artikel-Nr.:11686
• Hematoxylin acid according to MAYER, Artikel-Nr.:10231

colouring protocol

instruction manual

Technical informations

specifications

Instructions / Protocol / Recommendations

Verwendung:

The PAS (Periodic Acid-Schiff) reaction is one of the most common staining in histology. The PAS staining kit is suitable for the detection of 1,2-glycogen and mucosubstances. Frozen sections, thin section preparations, and kerosene sections are used for staining. The staining solution is intended exclusively for professional use as an in vitro diagnostic in the histology laboratory.

Prinzip:

PAS staining is a histochemical staining for the detection of carbohydrate-containing components, such as glycogen. Periodic acid is used in the first step of the staining. Oxidation of 1,2-glycogen to aldehyde groups occurs. \rIn the staining step with the sulfuric acid fuchsin contained in Schiff's reagent, a color complex is formed which appears pink to purple in the histological image. In combination with the hematoxylin, the nuclei are shown in blue color.

Verfahren:

PAS staining is a histochemical staining for the detection of carbohydrate-containing components, such as glycogen. Periodic acid is used in the first step of the staining. Oxidation of 1,2-glycogen to aldehyde groups occurs. \rIn the staining step with the sulfuric acid fuchsin contained in Schiff's reagent, a color complex is formed which appears pink to purple in the histological image. In combination with the hematoxylin, the nuclei are shown in blue color.

Hazard and safety instructions