Staining Kit: MSB-Lendrum Staining
Weigert stock solution A and B are used to prepare Weigert iron hematoxylin, which is responsible for staining cell nuclei, giving them a blue-black color.
Martius yellow and crystal ponceau are dyes used in combination with phosphotungstic acid to visualize fibrin and erythrocytes. In this process, fibrin appears in different colors depending on its age: young fibrin is red, older fibrin is yellow, and very old fibrin appears blue. Erythrocytes are stained yellow.
Methyl blue is another dye used to stain muscle fibers, giving them an intense blue color.
The different types of fibers, such as elastic fibers and collagen, appear in different colors, allowing them to be differentiated from one another. Extracellular matrix components such as glycoproteins and proteoglycans are also differentiated, allowing detailed analysis of the tissue.
The MSB-Lendrum staining method is particularly suitable for the study of inflammatory processes, thrombus formation, necrotic tissue areas and for the differentiated visualization of fiber types and extracellular matrix components.
Article no.: 12076
Staining of tissue samples
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product information
Components of this kit:
• WEIGERT stock solution A, Artikel-Nr.:10225A
• WEIGERT stock solution B, Artikel-Nr.:10225B
• Martius Yellow 0.5 %, Artikel-Nr.:11457
• Crystal Ponceau Solution, Artikel-Nr.:11454
• Phosphortungstic Acid 1 %, Artikel-Nr.:10318
• Methyl Blue 1 %, aqueous, Artikel-Nr.:11460
• Acetic Acid 10 %, Artikel-Nr.:13431
Instructions / Protocol / Recommendations
Verwendung:
The MSB-Lendrum staining kit is an in vitro diagnostic method for trichrome staining of tissue samples, allowing differential visualization of fibrin, erythrocytes, muscle fibers, collagen and other extracellular components. The method is particularly useful in the study of inflammatory processes, thrombus formation, necrotic tissue areas, and differential visualization of fiber types and extracellular matrix components. The various dyes in the kit provide detailed and high-contrast visualization of tissue components, enabling precise analysis and supporting diagnostics in various disease conditions.
Prinzip:
The MSB-Lendrum staining kit consists of various dyes and acids that specifically bind to different tissue or cell structures. Weigert stock solutions A and B are used to stain cell nuclei, Martius yellow and crystal ponceau stain connective tissue fibers, phosphotungstic acid binds to erythrocytes, methyl blue stains acidic mucopolysaccharides and acetic acid serves as a buffer. This combination allows detailed visualization of tissue structure and can be used in in vitro diagnostics to examine different types of tissue.
Verfahren:
The MSB-Lendrum staining kit consists of various dyes and acids that specifically bind to different tissue or cell structures. Weigert stock solutions A and B are used to stain cell nuclei, Martius yellow and crystal ponceau stain connective tissue fibers, phosphotungstic acid binds to erythrocytes, methyl blue stains acidic mucopolysaccharides and acetic acid serves as a buffer. This combination allows detailed visualization of tissue structure and can be used in in vitro diagnostics to examine different types of tissue.
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