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Staining Kit: MOVAT’s Pentachrom after VERHOEFF

The Movat Pentachrome staining kit according to Verhoeff is a histological staining method that can visualize five different tissue components simultaneously. This method is based on the Verhoeff stain for visualizing elastic fibers and was developed by Dr. Henry Zoltan Movat to visualize additional components in different colors.

The Movat Pentachrome stain shows the following tissue components:

1. Elastic fibers: black (by Verhoeff elastin stain)
2. Collagen: yellow (by True Saffron - Safran du Gâtinais)
3. Basic substance (mucopolysaccharides): blue (by Alcian blue)
4. Muscle fibers: red (by Brilliant crocein acid fuchsin solution)
5. Erythrocytes: yellow-orange (by Brilliant crocein acid fuchsin solution)

The kit consists of different solutions used in a specific order to obtain the desired staining results. The staining is done with 2% phosphotungstic acid. The use of the Movat Pentachrome staining kit according to Verhoeff requires precise protocols and careful handling to ensure optimal staining results. This method is particularly useful for the analysis of vascular wall layers, especially in the study of atherosclerosis, vascular abnormalities, or inflammatory changes.

Cartilage tissue is stained blue-green to dark green by metachromatic effects. Collagenous tissue (light yellow) can be differentiated from young osteoid (dark yellow); depending on fixation, osteon structures (reddish) are labeled. Muscle tissue, cytoplasm (red) and cell nuclei (blue-black) are differentiated. Modification according to Verhöff allows differentiation of elastic fibers (black), myelin sheaths (black-brown) and more intense nuclear staining.

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Article no.: 12061

Staining cartilage & bone

  •   Container size and order number Net price Amount
    Staining Kit: MOVAT’s Pentachrom after VERHOEFF - 100 ml
    Order number: 12061.00100
    203,97 €
    Staining Kit: MOVAT’s Pentachrom after VERHOEFF - 250 ml
    Order number: 12061.00250
    389,76 €
    Staining Kit: MOVAT’s Pentachrom after VERHOEFF - 500 ml
    Order number: 12061.00500
    805,13 €
    Staining Kit: MOVAT’s Pentachrom after VERHOEFF - 1.000 ml
    Order number: 12061.01000
    1.540,91 €

Important Information

UN-Nummer: siehe Einzelprodukte

Warning labels:

Lagerung: siehe Einzelprodukte


product information

Components of this kit:
• Alcian blue 1 % (pH 2,5 in acetic acid), Artikel-Nr.:12696
• VERHOEFF’s Stock Solution A, Artikel-Nr.:10402A
• VERHOEFF’s Stock Solution B, Artikel-Nr.:10402B
• VERHOEFF’s Stock Solution C, Artikel-Nr.:10402C
• Iron(III) Chloride 1 %, Artikel-Nr.:10174
• Brilliant Crocein - Acid Fuchsine, Artikel-Nr.:10156
• Phosphortungstic Acid 2 %, Artikel-Nr.:10321
• Safron du Gatinais, alcoholic, Artikel-Nr.:10369
• Acetic Acid 12 %, Artikel-Nr.:13827

colouring protocol

instruction manual

Technical informations

specifications

Instructions / Protocol / Recommendations

Verwendung:

The Movat Pentachrome staining kit according to Verhoeff is a histological staining method for in vitro diagnostics that enables the simultaneous visualization of five different tissue components. It is particularly used for the analysis of vascular wall layers, cartilage and bone tissue, as well as for the investigation of atherosclerosis, vascular abnormalities and inflammatory changes. Through precise protocols and careful handling, the method shows elastic fibers, collagen, ground substance, muscle fibers and erythrocytes in different colors, which allows for a differentiated analysis of the tissue under examination.

Prinzip:

The functional principle of the VERHÖFF MOVAT Pentachrome Staining Kit is based on the selective binding of its components to specific tissue or cell structures to visualize different structures in a sample. Alcian blue binds to acidic glycosaminoglycans, while VERHÖFF stock solutions A, B and C stain collagen and elastic fibers. Ferric chloride serves as an oxidizing agent, Brilliant crocein acid fuchsin stains cytoplasm and muscle, phosphotungstic acid binds to basement membranes, and Safron du Gatinais highlights cell nuclei. Acetic acid is used as a solvent and for pH regulation. The combination of these stains allows detailed visualization of various tissue and cell structures in in vitro diagnostics.

Verfahren:

The functional principle of the VERHÖFF MOVAT Pentachrome Staining Kit is based on the selective binding of its components to specific tissue or cell structures to visualize different structures in a sample. Alcian blue binds to acidic glycosaminoglycans, while VERHÖFF stock solutions A, B and C stain collagen and elastic fibers. Ferric chloride serves as an oxidizing agent, Brilliant crocein acid fuchsin stains cytoplasm and muscle, phosphotungstic acid binds to basement membranes, and Safron du Gatinais highlights cell nuclei. Acetic acid is used as a solvent and for pH regulation. The combination of these stains allows detailed visualization of various tissue and cell structures in in vitro diagnostics.

Hazard and safety instructions

Signal Word 2: flamme, exclam, ,
Warning labels: