Staining Kit: Congo Red after HIGHMAN
The kit contains the Congo Red solution, which consists of Congo Red dye in an aqueous solution. When Congo Red stain is used, the dye selectively binds to amyloid deposits, which then become visible under a light microscope in a characteristic red-orange hue. Another important feature of this stain is birch fringence, in which the stained amyloid deposits show a greenish and/or red-blue hue under polarized light.
Highman's Congo Red staining kit provides an effective and reliable method for identifying amyloid deposits in various tissues. Staining is of great importance in the study of amyloidosis and other diseases associated with the accumulation of amyloid proteins, and supports histological and histopathological analysis and diagnosis.
Article no.: 11084
Staining of tissue samples
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product information
Components of this kit:
• Hematoxylin acid according to MAYER, Artikel-Nr.:10231
• Congo Red 0.5 % in Ethanol 50 %, Artikel-Nr.:11794
• Alkaline Alcohol (with KOH), Artikel-Nr.:12437
• SCOTT’s Solution, Artikel-Nr.:11192
Instructions / Protocol / Recommendations
Verwendung:
The Congo Red staining kit according to Highman is used for the histological and histopathological examination of amyloid deposits in tissue samples. It allows visualization and identification of amyloid, an abnormal protein deposited in organs and tissues in diseases such as Alzheimer's disease and systemic amyloidosis. The characteristic red-orange staining and birch fluorescence under polarized light facilitate analysis and diagnosis in the study of amyloidosis and other diseases associated with amyloid protein deposition.
Prinzip:
The Congo Red staining kit according to HIGHMAN uses various solutions to selectively stain tissue or cell structures in in vitro diagnostics. Hematoxilin binds to basic (positively charged) structures such as cell nuclei and appears blue-violet. Congo red, an azo dye, binds to amyloid deposits and shows them in red. Alkaline alcohol is used to differentiate and decolorize excess hematoxilin, while Scott's solution is a bluing reagent that stabilizes hematoxilin staining. Overall, the kit provides differential visualization of tissue and cellular structures for analysis and diagnosis.
Verfahren:
The Congo Red staining kit according to HIGHMAN uses various solutions to selectively stain tissue or cell structures in in vitro diagnostics. Hematoxilin binds to basic (positively charged) structures such as cell nuclei and appears blue-violet. Congo red, an azo dye, binds to amyloid deposits and shows them in red. Alkaline alcohol is used to differentiate and decolorize excess hematoxilin, while Scott's solution is a bluing reagent that stabilizes hematoxilin staining. Overall, the kit provides differential visualization of tissue and cellular structures for analysis and diagnosis.
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