Staining Kit: GROCOTT’s Staining for Fungi
The solutions contained in the staining kit are:
1. Chromic acid 5%: Used to oxidize and remove reducing substances in the tissue to allow better silver binding.
2. Kit: Silver Methenamine Borax: Contains silver nitrate and methenamine, which play a role in silver impregnation.
3. Sodium Disulfite 1%: Reducing agent that promotes the reduction of silver ions to elemental silver, which attaches to fungal structures.
4. Gold Chloride 0.1%: Used to tint and improve contrast in staining.
5. Sodium thiosulfate 5%: Removes excess gold chloride and stabilizes the dyeing.
6. Light Green 0.2 % (GOLDNER III): Counterstains background tissue to better identify and delineate fungal structures.
Together, these components of the GROCOTT staining kit enable specific staining of fungal spores and clear delineation from surrounding tissue structures in histological sections.
Article no.: 12053
Staining of tissue samples
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product information
Components of this kit:
• Chromic Acid 5 %, Artikel-Nr.:11697
• Kit: Silver Methenamin Borax, Artikel-Nr.:12512
• Sodium Disulfite 1%, Artikel-Nr.:11800
• Goldchloride 0.1 %, Artikel-Nr.:11134
• Sodium Thiosulfate 5 %, Artikel-Nr.:10288
• Light Green 0.2 % (GOLDNER III), Artikel-Nr.:10267
Instructions / Protocol / Recommendations
Verwendung:
The GROCOTT staining kit is used for the identification and visualization of fungal spores in histological tissue samples, especially for in vitro diagnostics. It is based on GROCOTT-Gomori methenamine silver staining (GMS), a silver impregnation method. The solutions included in the kit allow specific staining of fungal structures, their detection and differentiation from surrounding tissue structures, as well as improved contrast and stabilization of the stain. This makes it an important tool for the diagnosis of fungal infections in tissue samples.
Prinzip:
The GROCOTT staining kit is used for the detection of fungal spores in tissue or cell structures in in vitro diagnostics. The various components of the kit bind selectively to specific structures in the cells. Chromic acid is used to oxidize cell structures, while silver methenamine borax acts as a reducing agent to reduce silver ions to produce visible staining. Sodium disulfite removes excess silver, gold chloride intensifies the staining, and sodium thiosulfate stabilizes the result. Light green serves as a contrast dye to make the fungal spores more visible. Overall, the kit enables specific and sensitive detection of fungal spores in the material under investigation.
Verfahren:
The GROCOTT staining kit is used for the detection of fungal spores in tissue or cell structures in in vitro diagnostics. The various components of the kit bind selectively to specific structures in the cells. Chromic acid is used to oxidize cell structures, while silver methenamine borax acts as a reducing agent to reduce silver ions to produce visible staining. Sodium disulfite removes excess silver, gold chloride intensifies the staining, and sodium thiosulfate stabilizes the result. Light green serves as a contrast dye to make the fungal spores more visible. Overall, the kit enables specific and sensitive detection of fungal spores in the material under investigation.
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