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Staining Kit: Iron Staining after HALE

This staining kit for iron Hale staining provides a reliable method for visualizing acidic mucosubstances that are indicated by the reaction of colloidal iron ions with carboxyl and sulfate residues in blue. In this context, a Berliner blue reaction is used to differentiate chromophobe to light cell renal cell carcinoma.

The special suitability of the kit lies in its high sensitivity and selective detection of acidic muco substances. This enables precise differentiation and classification of renal cell carcinomas, a crucial factor for accurate diagnosis and selection of optimal therapy.

Chemically, the staining kit works with the specific reaction of colloidal iron ions and carboxyl and sulfate residues, resulting in blue staining. The subsequent Berliner blue reaction provides additional differentiation of carcinoma types.

The kit's effectiveness is evident in the way the iron Hale stain works. The accurate display of acidic mucosal substances provides a high level of detail that provides the basis for subsequent differentiation of carcinoma types. The ease of use and reproducible results make the kit a valuable tool in medical diagnostics and biomedical research.

Article no.: 13688

detection of iron

  •   Container size and order number Net price Amount
    Staining Kit: Iron Staining after HALE - 100 ml
    Order number: 13688.00100
    31,88 €
    Staining Kit: Iron Staining after HALE - 250 ml
    Order number: 13688.00250
    38,89 €
    Staining Kit: Iron Staining after HALE - 500 ml
    Order number: 13688.00500
    68,04 €
    Staining Kit: Iron Staining after HALE - 1.000 ml
    Order number: 13688.01000
    132,47 €

Important Information

UN-Nummer: siehe Einzelprodukte

Warning labels:

Lagerung: siehe Einzelprodukte


product information

Components of this kit:
• Colloidal Iron Solution (Stock Solution) after MUELLER, Artikel-Nr.:13674
• Acetic Acid 99 % (Glacial Acid), Artikel-Nr.:11998
• Potassium Ferrocyanide (II) 2 % (Yellow Prussiate), Artikel-Nr.:13306
• Hydrochloric Acid 2 %, Artikel-Nr.:13694
• Neutral Red, Artikel-Nr.:11683

colouring protocol

Technical informations

specifications

Instructions / Protocol / Recommendations

Verwendung:

The Iron HALE staining kit is used for in vitro diagnostics and allows precise examination and identification of acidic mucosubstances present in renal cell carcinomas. It uses colloidal iron ions that react with carboxyl and sulfate residues to stain muco substances blue. A Berlin blue reaction can then be used to differentiate chromophobe from light cell renal cell carcinoma. This method offers high sensitivity and selectivity, which contributes to accurate diagnoses and optimized treatment decisions. It is also easy to use and enables reproducible results in medical diagnostics and biomedical research.

Prinzip:

The functional principle of iron HALE staining is based on the binding of colloidal iron to tissue or cell structures. The acetic acid enables better binding of the iron, while potassium ferricyanide (II) leads to the formation of berliner blue, which serves as a visible dye. The hydrochloric acid stabilizes the reaction environment and neutral red serves as a contrast dye. This interaction of components allows selective staining of structures, such as cell nuclei or fibers, for easier visual examination and diagnostics in the laboratory during in vitro experiments.

Verfahren:

The functional principle of iron HALE staining is based on the binding of colloidal iron to tissue or cell structures. The acetic acid enables better binding of the iron, while potassium ferricyanide (II) leads to the formation of berliner blue, which serves as a visible dye. The hydrochloric acid stabilizes the reaction environment and neutral red serves as a contrast dye. This interaction of components allows selective staining of structures, such as cell nuclei or fibers, for easier visual examination and diagnostics in the laboratory during in vitro experiments.

Hazard and safety instructions

Signal Word 2: flamme, acid_red, ,
Warning labels: