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Staining Kit: Alcian Blue-PAS Staining

The Alcian Blue PAS staining kit is a useful tool in histology and medical diagnostics for visualizing neutral polysaccharides and acidic mucosubstances in connective and supporting tissue. Neutral polysaccharides are given a red color by application, while acidic mucosubstances appear bright blue. Surrounding connective and supporting tissues acquire a pink background staining.

The special suitability of this kit lies in the differentiated visualization of tissue components. It allows differentiated analysis of tissue sections, especially in the diagnosis of diseases associated with altered composition or structure of connective tissue.

The chemical composition of the kit includes alcian blue, periodic acid, Schiff's reagent, and hematoxylin. Alcian blue binds to acidic mucosubstances and gives them a blue color. Periodic acid oxidizes neutral polysaccharides, which subsequently interact with Schiff's reagent and are colored red. Hematoxylin is used as a counterstain and produces the pink background.

The operation of the kit is based on the specific chemical interaction of the included reagents with the target structures in the tissue. This specific binding enables differential staining and thus visual differentiation of the various tissue components.

Article no.: 11388

Detection of mucopolysaccharides

  •   Container size and order number Net price Amount
    Staining Kit: Alcian Blue-PAS Staining - 100 ml
    Order number: 11388.00100
    53,19 €
    Staining Kit: Alcian Blue-PAS Staining - 250 ml
    Order number: 11388.00250
    89,26 €
    Staining Kit: Alcian Blue-PAS Staining - 500 ml
    Order number: 11388.00500
    176,48 €
    Staining Kit: Alcian Blue-PAS Staining - 1.000 ml
    Order number: 11388.01000
    338,60 €
    Staining Kit: Alcian Blue-PAS Staining - 2.500 ml
    Order number: 11388.02500
    776,60 €

Important Information

UN-Nummer: siehe Einzelprodukte

Lagerung: siehe Einzelprodukte


product information

Components of this kit:
• Alcian blue 1 % (pH 2,5 in acetic acid), Artikel-Nr.:12696
• Periodic Acid 1 %, Artikel-Nr.:11415
• SCHIFF’s Reagent, Artikel-Nr.:11686
• Hematoxylin after GILL - III, Artikel-Nr.:11773

colouring protocol

instruction manual

Technical informations

specifications

Instructions / Protocol / Recommendations

Verwendung:

Alcian blue-PAS staining is a combination stain that uses the PAS reaction and the reaction of Alcian blue to visualize various carbohydrates depending on pH and electrolyte concentration. PAS staining is performed before staining with Alcian blue to stain neutral mucins, neutral glycoproteins and glycogen.\rAlcian blue PAS staining is particularly important in the diagnosis of lung tumors and lung metastases, as well as diseases of the gastrointestinal tract, but is also used for other carcinomas and disease patterns.

Prinzip:

Alcian blue is used in histology to selectively stain acid mucosubstances (acid mucins and sulfated glycosaminoglycans such as hyaluronic acid or chondroitin sulfate) at low pH (1.0 to 2.5). Depending on the pH of the staining solution, specific mucins can be detected. Another method of selective imaging, is to vary the electrolyte concentration. As the ionic strength increases, staining with Alcian blue is prevented, allowing differentiation of differently sulfated mucopolysaccharides as well as heparins, heparin sulfates and keratin sulfates.

Verfahren:

Alcian blue is used in histology to selectively stain acid mucosubstances (acid mucins and sulfated glycosaminoglycans such as hyaluronic acid or chondroitin sulfate) at low pH (1.0 to 2.5). Depending on the pH of the staining solution, specific mucins can be detected. Another method of selective imaging, is to vary the electrolyte concentration. As the ionic strength increases, staining with Alcian blue is prevented, allowing differentiation of differently sulfated mucopolysaccharides as well as heparins, heparin sulfates and keratin sulfates.

Hazard and safety instructions