Eosin 0.5% in methanol is a common staining solution used in histological and histopathological examination of tissue specimens. Compared to aqueous eosin solutions, methanolic eosin solution provides improved tissue dehydration and faster staining due to improved penetration of methanol.

Eosin is a red dye commonly used as a counterstain to hematoxylin to highlight various tissue components. The eosin solution stains cytoplasmic structures, extracellular matrix and other tissue components in a pink-orange hue, while hematoxylin stains cell nuclei blue-violet. This combination of dyes allows clear differentiation and identification of various cell types and structures within tissue samples.

The 0.5% methanolic eosin solution provides a reliable and effective way to stain tissue samples and supports histological and histopathological analysis and diagnostics in the study of cell structures and functions. The solution is particularly suitable for applications where rapid and efficient dehydration and staining are required.