Aniline Blue - Acid Fuchsin - Orange G represents a special staining solution used in histological examinations. The dyes it contains have different affinities for different tissue components, allowing for differentiated staining.

When used, aniline blue binds specifically to collagen fibers, orange G binds preferentially to cell nuclei, and acid fuchsin binds to muscle tissue. The interaction of these dyes provides a multi-layered and high-contrast visualization of tissue composition.

In the specific case of AFOG/SFOG staining according to Mallory & Cason, this solution is used for differential visualization of muscle tissue, collagen and cell nuclei. The result is a color-differentiated representation of tissue structure that enables precise analysis and diagnosis.

The solution is dissolved in water and hydrochloric acid, which ensures optimal dye distribution and efficient penetration of tissue sections.