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Immunhistochemie etablierte Antikörper und Nachweise

Establishments for immunohistochemistry / immunohistology

Are you planning to use new antibodies for the specific presentation of proteins and for tissue analysis? With us you have a reliable partner for the selection of suitable antibodies and the establishment of routine processing.

The professional execution of mostly time-consuming and personnel-intensive establishment studies for diagnostics and basic research in medicine, zoology and botany is one of our areas of expertise. In this way we support you in immunohistological routine work as well as in long-term research projects.

Our experienced team quickly and reliably establishes the immunoassays of your choice on individual sample material, prepares the reference preparations necessary for the subsequent routine and develops reproducible work protocols for your further research work.

We have already established a large number of first antibodies in our routine (see tables below). We will be pleased to carry out establishment work for your requirements and then also carry out routine tests on your preparations.

Here are some examples:

  • Collagen I, II, III, IV, V, VI, VIII and XII
  • Osteopontin, Periostin, Fibronectin, Laminin, BSP and Elastin
  • Vasopressin, Oxytoxin, Angiotensin I/II, Angiotensin II AT1 & AT2 receptors
  • Bradykinin, Ki-67, anti-galactose alpha 1,3
  • CD4, CD31, FoxP3
  • Cytokeratins
  • Digoxigenin

Etablierte Erst-Antikörper und Immunnachweise (A-I)

Epitop

Wirt

Reaktivität

Anti-Cytrochrome 17A1 P450

rabbit

human

ASS1

rabbit

mouse, rat, human

Bradykinin

goat

mouse, rat, >human

BSP

rabbit

human

Catenin-β

goat

mouse, rat, human, Xenophus laevis, zebrafish 

CD138 (Syndecan)

rabbit

mouse, rat, human

CD138 (Syndecan)

mouse

human

CD19

rabbit

human, mouse, rat

CD31

rabbit

human, mouse

CD33

rabbit

human, mouse, rat

CD34

rabbit

human 

CD4 

mouse

human

CD44

rabbit

human

CD45

mouse

human

CD68

mouse

rat

CD68

rabbit

mouse

Col 1 

rabbit

bovine, porcine, sheep

Col 12 A1

rabbit

human

Col 2

rabbit

human, mouse, rat

Col 3

rabbit

mouse, rat, cow, human

Col 4

mouse

human

Col 4

rabbit

human, feline, canine, fish, mouse, rat, swine

Col 5 

rabbit

rat, mouse, bovine

Col 6

rabbit

mouse, rat, rabbit, cow, dog, human, pig

Col 8A1

rabbit

human, mouse, rabbit

Col10

rabbit

human

CREB

rabbit

human, mouse, rat, monkey, Drosophila melanogaster

Cytokeratin 10

mouse

rat, mouse, human

Cytokeratin 14

mouse

dog, rat, cat, mouse, human, rhesus monkey

Cytokeratin 5 & 6

mouse

human

Cytokeratin Pan Type I/II 

mouse

rat, primate, sheep, zebrafish, fish, mouse, human, chicken

Elastin

guinea pig

human

Elastin

rabbit

human 

Fascin

mouse

human (gerbil, rat)

Fibrinogen

mouse

human

Fibronektin

goat

mouse, rat, human

Galactose alpha

mouse

rabbit, human, mouse, rat, porcine, baboon

GFLV

/

/

Hif-1 α

goat

human, mouse, rat, equine, canine, bovine, porcine

HLA-A,B,C

mouse

human           (mouse & rat not tested) 

IKK-i

rabbit

mouse, rat, human

 

Etablierte Erst-Antikörper und Immunnachweise (K-Z)

Epitop

Wirt

Reaktivität

Ki67

rabbit

human, mouse

Ki67

rabbit

human

Laminin

rabbit

mouse, rat, dog, human, pig, frog

LAMP-1 (D2D11)

rabbit

human, rat

LYVE-1

goat

rat, mouse

LYVE-1

rat

mouse

Mouse IgG1 isotype control

mouse

 

Mouse IgG1 isotype control (Biotin)

mouse

 

MSY2

goat

mouse

Neurfilament (SMI312)

  

Neurofilament

mouse

human

Neurofilament L

chicken

human, bovine, mouse, rat

NRP1 (Neuropilin)

rabbit

human, mouse, rat

Oncostatin M

mouse

human

Oncostatin M

rabbit

human

OSMR β

mouse

human

Osteocalcin

mouse

human, rat 

Osteopontin

rabbit

human

OTC

rabbit

human, mouse, rat, cow, dog, pig, sheep

Oxytocin

rabbit

human, hamster, mouse, rat, rabbit, sheep

p21 (12D1)

rabbit

human, monkey, dog

p63

rabbit

mouse, rat, human

PCNA

mouse

mouse, rat, chicken, pigeon, human, pig, fruitfly, monkey, zebrafish, dogfish/catshark, Thornback ray (predicted: cow)

PECAM-1

rabbit

mouse, rat, >human

Periostin

rabbit

birds, chicken, human, mouse, rat, mammals

Phospho-Histone H2AX

rabbit

mouse, rat, human, monkey

PJA-2

mouse

mouse, rat, human, horse, dog, bovine, pig

S100B

rabbit

human, rat (mouse)

S100B

rabbit

mouse, rat, human, goat, zebrafish, Macaque monkey

Synaptophysin1

guinea pig

human, rat, mouse, hamster, cow, chicken, frog

Trimethylhiston H3 (Lys9)

rabbit

human, rat, mouse, monkey

Vasopressin

rabbit

human, mouse, rat, rabbit, sheep

Vimentin

rabbit

human

Etablierte Zweit-Antikörper

Wirt

Reaktivität

Filter

Farbstoff

goat

anti-chicken

  

goat

anti-guinea pig

  

goat

anti-mouse

  

goat

anti-mouse

  

goat

anti-rabbit

  

horse

anti-goat

  

horse

anti-mouse

  

horse

anti-mouse

  

rabbit

anti-goat

  

rabbit

anti-rat

  

rabbit

anti-sheep

  

chicken

anti-goat

AlexaFluor 647

Cy5

donkey

anti-rabbit

AlexaFluor 568

TexasRed

goat

anti-chicken

AlexaFluor 647

Cy5

goat

anti-guinea pig

AlexaFluor 568

TexasRed

goat

anti-mouse

AlexaFluor 555

Cy3

goat

anti-mouse

AlexaFluor 555

Cy3

goat

anti-mouse

AlexaFluor 568

TexasRed

goat

anti-mouse

AlexaFluor 647

Cy5

goat

anti-mouse

AlexaFluor 750

Cy7

goat

anti-mouse

DyLight 550

Cy3

goat

anti-rabbit

AlexaFluor 405

att. DAPI

goat

anti-rabbit

AlexaFluor 488

FITC

goat

anti-rabbit

AlexaFluor 647

Cy5

goat

anti-rabbit

DyLight 488

FITC

rabbit

anti-goat

AlexaFluor 568

TexasRed

rabbit

anti-mouse

AlexaFluor 488

FITC

Enzyme und Substrate

enzyme

substrate

colour of precipitation

horseradish peroxidase (HRP)

DAB (3,3‘-Diaminobenzidine)

brown

 

4-CN (4-Chlor-1-Naphthol)

blue-black

 

AEC (3-Amino-9-Ethylcarbazol)

red

 

Hanker-Yates-Reagent

(p-Phenylendiamin-HCL and Pyrocatechol)

purple-brown

 

TMB (3,3‘,5,5‘-Tertamethylbenzidin) 

dark blue

 

Α-Naphtol/Pyronin

red-purple

alkaline phosphatase (AP) 

Naphthol AS-Phosphat +

 
 

fast red TR

red

 

fast blue BBN

blue 

 

fast red violet LB

violet

 

Bromchlorindoylphosphat (BCIP) und Nitrotetrazoliumblau (NBT)

blue 

glucoseoxidase (GO)

t-NBT und m-PMS (Nitrotetrazoliumblauchloride and Phenazinmethosulfat)

blue-purple