Veronal buffer, also known as barbiturate buffer, is a type of biological buffer used in biochemical and molecular biology applications. The specific role of the buffer is to keep the pH of a solution constant, in this case at pH 8.5.

Veronal is the trade name for barbiturate, a nervous system depressant used in buffers for its buffering capacity in the pH range of 8.6 to 9.6. At a pH of 8.5, the veronal buffer is used in the slightly basic range.

The uses of the pH 8.5 veronal buffer are many and include immunostaining, enzyme reactions, and various types of biochemical analyses where a stable pH is critical.

Please note that the exact concentration of Veronal and other components in the buffer may vary depending on the specific experiment or procedure. Therefore, it is important that the buffer be properly formulated to ensure accurate and reliable results.

Because veronal is potentially toxic, care should be taken when handling it. It is also important to note that barbiturates are controlled substances and their use and handling are regulated by law.