With a pH of 8.6, the Veronal Buffer provides a stable alkaline environment used in numerous life science and medical applications. Particularly in electrophoresis, the buffer enables reliable separation of biomolecules by controlling the ionic strength of the solution and thus influencing the migration speed of the molecules.

The Veronal Buffer shows particular suitability in serum protein electrophoresis, where it facilitates the separation of proteins according to their electrical charge. It is also widely used in immunoelectrophoresis, where it provides an optimal environment for antigen-antibody reactions.

Chemically, the buffer consists of a mixture of sodium 5,5-diethylbarbiturate, sodium acetate and citric acid dissolved in distilled water. In this process, the diethyl barbiturate creates a stable pH environment and the citric acid and sodium acetate serve as buffer components that help maintain the desired pH.

The functioning of the veronal buffer is based on its ability to keep the pH of a solution stable, even when additional acids or bases are added. This makes it an indispensable component in numerous laboratory processes where a stable pH environment is required.