Sudan III in glacial acetic acid consists of a solution of Sudan III, a fat-soluble dye dissolved in a mixture of acetic acid and denatured ethanol. The solvent in this product is acetic acid, which makes up the largest portion.

The main uses of Sudan III in glacial acetic acid are in histology and food analysis. The dye is particularly suitable for staining lipid-containing structures, such as fat droplets, in tissue sections and cell preparations. In food analysis, Sudan III is used for the detection of fat contaminants in food.

The chemical mode of operation is based on the lipophilic property of Sudan III, which selectively accumulates in lipid-containing structures, making them visible. The acetic acid and ethanol serve as solvents to keep the dye stable in the solution and allow uniform staining of the samples under investigation.

The special suitability of Sudan III in glacial acetic acid lies in the highly specific staining of lipid-containing structures, which allows them to be clearly distinguished from other cellular components. The solution is suitable for the examination of both animal and plant samples and thus offers a wide range of applications in research and analysis.