Sudan III 1 %, alcoholic, is used in medical and histological diagnostics for staining tissue samples. Due to the specific affinity of Sudan III for fats, fatty tissue and particles are marked and made visible. Here, the property of Sudan III as a lipophilic dye comes into play, which attaches to fatty acids and thus contributes to visualization. The solution is dissolved in an alcohol-water mixture, which facilitates penetration into the tissue and allows uniform staining. The result is a precise and well-defined visualization of adipose tissues that supports reliable analysis and diagnosis. It should be noted that the application of Sudan III to specific tissue types may result in different staining patterns.