Sudan III 0.1 % in glycerol-ethanol is an efficient laboratory chemical for staining tissue samples. It is ideally suited for the detection of lipids and fatty acids in biological samples, especially for histological and cytological examinations. By using this staining method, structures in tissue samples can be better identified and analyzed.

The chemical and physical processes involved in using Sudan III solution are based on the affinity of Sudan III for fat cells within tissue samples. The dye interacts specifically with lipids and fatty acids, resulting in specific staining of the cells and structures of interest. Ethanol and glycerol result in good solubility of the Sudan III dye and ensure homogeneous distribution within the tissue.

The application of this laboratory chemical enables clear delineation and identification of fat-rich cells and structures, as well as improved visualization of the samples under investigation. Furthermore, precise qualitative and quantitative analyses of the distribution and concentration of lipids and fatty acids in the samples are possible.