The hydrochloric acid-alcohol solution (0.8 % / 96 %) is particularly known for its differentiating effect in histology, which helps to optimize staining results. By selectively applying the solution to histological specimens, staining can be selectively concentrated on specific cell structures. This improves contrast and enables more accurate microscopic analysis of tissue specimens in medical diagnostics and life sciences.

Another aspect of this solution's special suitability is its ability to remove stains from specimens when exposed for extended periods of time. This can reverse unwanted or excessive staining and improve the quality of histological specimens.

Chemical properties of the hydrochloric acid-alcohol solution result from the combination of ethanol and hydrochloric acid. The solution has a slightly acidic nature, which supports the differentiating effect. The reactions that occur during use include the interaction of the acidic components with basic structures in tissue specimens, enabling selective staining or destaining.

The operation of the hydrochloric acid-alcohol solution is based on its ability to selectively interact with various cell structures in histological specimens. The chemical reactions that occur during the staining and destaining process allow effective separation and identification of cells and tissue components, contributing to better visualization and analysis.