HANSEN’s Picric Fuchsine
Fuchsin is a synthetic dye widely used in histology because of its ability to stain various tissue structures. In Hansen's picrofuchsin solution, fuchsin contributes to the staining of cell structures, especially nuclei and cytoplasm.
Picric acid, a yellow acid, stains connective tissue yellowish in this solution. The combination of fuchsin and picric acid allows differential visualization of tissue components such as collagen fibers and elastin. The acetic acid in Hansen's picrofuchsin solution serves as an acidic solvent that allows the dye to penetrate the tissue and improve staining results.
Hansen's picrofuchsin solution can be used in combination with other staining methods to allow better differentiation and analysis of various tissue components. For example, Hansen's picrofuchsin staining can be used in conjunction with hematoxylin staining. Hematoxylin stains the cell nuclei blue, while Hansen's picrofuchsin stains the connective tissue in yellowish tones. This combination of stains allows clearer differentiation of cell structures and extracellular matrix components in tissue samples, facilitating analysis of tissue morphology and structure under the microscope.
Article no.: 10345
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Lagerung: 15 … 25 °C
Haltbarkeit: 24 Monate
product information
Relevant Incredients:
• Picric acid (C.I.: 10305)
• Acid Fuchsine (C.I.: 42685)
Instructions / Protocol / Recommendations
Verwendung:
Hansen's microfuchsin is a histological staining solution for tissue specimens, mainly used to visualize fibrous connective tissue such as collagen fibers. The combination of fuchsin, picric acid and acetic acid in this solution allows differential staining of different tissue components, facilitating analysis of tissue morphology and structure under the microscope. The solution can also be combined with other staining methods, such as hematoxylin staining, to allow better differentiation and analysis of cell structures and extracellular matrix components.
Prinzip:
According to HANSEN, microfuchsin is a staining method in histology and in vitro diagnostics that uses a mixture of picric acid, acid fuchsin and acetic acid. Picric acid binds to basic structures in tissues and cells, such as proteins and nucleic acids, while acid fuchsin binds to acidic structures, such as glycosamine glycans. The principle of operation is based on the interaction of picric acid and acid fuchsin, which leads to differentiation of tissue or cell structures in the stained object. The addition of acetic acid adjusts the pH of the solution to ensure optimal conditions for the staining reaction.
Verfahren:
According to HANSEN, microfuchsin is a staining method in histology and in vitro diagnostics that uses a mixture of picric acid, acid fuchsin and acetic acid. Picric acid binds to basic structures in tissues and cells, such as proteins and nucleic acids, while acid fuchsin binds to acidic structures, such as glycosamine glycans. The principle of operation is based on the interaction of picric acid and acid fuchsin, which leads to differentiation of tissue or cell structures in the stained object. The addition of acetic acid adjusts the pH of the solution to ensure optimal conditions for the staining reaction.
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