Sodium acetate-acetic acid buffer
Sodium acetate acetic acid buffer is a solution used in various applications, including molecular biology, biochemistry and analytical chemistry. Frequently, this buffer is used to stabilize the pH in DNA and protein extractions and in electrophoresis of nucleic acids and proteins.
The special suitability of this buffer system results from the combination of sodium acetate and acetic acid, which together cover a pH range of approximately 3.6 to 5.6. This allows the optimization of reaction conditions for enzymatic processes and the stabilization of biomolecules.
The chemical operation of the sodium acetate-acetic acid buffer is based on the equilibrium between the conjugate acid-base pairing of acetic acid and its corresponding sodium salt, sodium acetate. By adjusting the concentrations of the two components, the desired pH can be set and the buffer capacity optimized.
Compared to other buffer systems, the sodium acetate-acetic acid buffer offers advantages in terms of stability and adaptability of the pH range. These properties make it a versatile and reliable buffer that can be used in various scientific and technical applications.
The special suitability of this buffer system results from the combination of sodium acetate and acetic acid, which together cover a pH range of approximately 3.6 to 5.6. This allows the optimization of reaction conditions for enzymatic processes and the stabilization of biomolecules.
The chemical operation of the sodium acetate-acetic acid buffer is based on the equilibrium between the conjugate acid-base pairing of acetic acid and its corresponding sodium salt, sodium acetate. By adjusting the concentrations of the two components, the desired pH can be set and the buffer capacity optimized.
Compared to other buffer systems, the sodium acetate-acetic acid buffer offers advantages in terms of stability and adaptability of the pH range. These properties make it a versatile and reliable buffer that can be used in various scientific and technical applications.
Article no.: 13365
Preparation of buffer solutions
product information
Relevant Incredients:
• Sodium acetat
• Acetic acid 99%