Kit: Potassium Ferrocyanide (II) Hydrochloric Acid Solution
The potassium hexacyanoferrate(II) hydrochloric acid solution kit is specially designed for histology application in Berlin blue staining. The main components of the kit are:
1. Potassium hexacyanoferrate(II) solution: an aqueous solution of potassium hexacyanoferrate(II) (K_4[Fe(CN)_6]), also known as potassium ferrocyanide. In Berlin blue staining, it reacts with ferric ions to form the characteristic Berlin blue complex (ferriferrocyanide).
2. Hydrochloric acid solution: an aqueous solution of hydrochloric acid (HCl) that serves as a source of acid and promotes the formation of the Berlin blue complex.
Berlin blue staining is an important technique in histology to visualize iron deposits in tissue sections. These deposits are typically present in medical conditions such as hemochromatosis, hemoglobinopathies, and other diseases associated with iron overload.
The chemical reaction responsible for the formation of the Berlin blue complex can be represented as follows:
Fe³⁺ + K₄[Fe²⁺(CN)₆] + 6H⁺ → Fe³⁺[Fe²⁺(CN)₆] + 6K⁺ + 3H₂
In other applications, potassium ferricyanide(II) is used as a reducing agent, for the synthesis of various chemical compounds, and as a component of electrolyte solutions in electrochemistry. Hydrochloric acid is a widely used acid in many chemical processes and reactions, including pH adjustment, hydrolysis of compounds, and dissolution of metals and minerals.
This kit is designed for professionals who need a reliable and efficient source of potassium ferricyanide(II) and hydrochloric acid to perform their staining and chemical applications.
1. Potassium hexacyanoferrate(II) solution: an aqueous solution of potassium hexacyanoferrate(II) (K_4[Fe(CN)_6]), also known as potassium ferrocyanide. In Berlin blue staining, it reacts with ferric ions to form the characteristic Berlin blue complex (ferriferrocyanide).
2. Hydrochloric acid solution: an aqueous solution of hydrochloric acid (HCl) that serves as a source of acid and promotes the formation of the Berlin blue complex.
Berlin blue staining is an important technique in histology to visualize iron deposits in tissue sections. These deposits are typically present in medical conditions such as hemochromatosis, hemoglobinopathies, and other diseases associated with iron overload.
The chemical reaction responsible for the formation of the Berlin blue complex can be represented as follows:
Fe³⁺ + K₄[Fe²⁺(CN)₆] + 6H⁺ → Fe³⁺[Fe²⁺(CN)₆] + 6K⁺ + 3H₂
In other applications, potassium ferricyanide(II) is used as a reducing agent, for the synthesis of various chemical compounds, and as a component of electrolyte solutions in electrochemistry. Hydrochloric acid is a widely used acid in many chemical processes and reactions, including pH adjustment, hydrolysis of compounds, and dissolution of metals and minerals.
This kit is designed for professionals who need a reliable and efficient source of potassium ferricyanide(II) and hydrochloric acid to perform their staining and chemical applications.
Article no.: 12566
Iron detection
Important Information
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Lagerung: siehe Einzelprodukte
product information
Components of this kit:
• Hydrochloric Acid 5 % for Iron Detection, Artikel-Nr.:11632
• Potassium Ferrocyanide (II) 5 % (Yellow Prussiate), Artikel-Nr.:11333
Hazard and safety instructions
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