Kit: Developer Solution for WARTHIN-STARRY Silver Staining
The kit's special suitability lies in its ability to visualize bacteria in complex tissue samples that are difficult to detect using conventional staining techniques. The gelatin solution, hydroquinone solution and silver nitrate solution together form the staining system that selectively stains bacteria and enables their identification.
The kit's chemical mode of operation is based on the reduction of silver nitrate by hydroquinone in the presence of gelatin. The reduced silver ions form dark silver particles that bind to the bacteria and make them visible under the microscope. The gelatin solution ensures uniform distribution of the silver particles and facilitates binding to the target structures.
Compared to other silver staining techniques, the Warthin-Starry kit offers the advantage of higher sensitivity and specificity in the detection of Helicobacter pylori and spirochetes. By using this kit, histologists can obtain detailed information about the distribution and morphology of these bacteria in tissue samples, which is important for the diagnosis and understanding of infectious diseases.
Article no.: 13324
Impregnation of fabric cuts
Important Information
Lagerung: 15 … 25 °C
product information
Components of this kit:
• Gelatine 5 %, Artikel-Nr.:13369
• Hydroquinone 0.15 %, Artikel-Nr.:13342
• Silver Nitrate 1 %, Artikel-Nr.:11180
instruction manual
Instructions / Protocol / Recommendations
Verwendung:
The Warthin-Starry stain is used for the histological detection of Heliobacter pylori in tissue samples using light microscopy. Various modifications of this stain are used for the targeted detection of other bacteria, such as Bacillus piliformis and spirochetes. Detection is usually carried out in samples of the surface epithelium of the gastric glands and gastric mucosa.
Prinzip:
The Warthin-Stary stain is a classic silver nitrate stain in which silver nitrate is reduced to metallic silver with the aid of a hydroquinone-containing developer. The reaction is stopped after a certain time by a rinsing step. As a result, the tissue is stained yellow-brown to golden-brown, while bacteria stand out in black. Cell nuclei take on a dark brown colour. The staining is stabilised by a final fixation reaction in sodium thiosulphate. If this step is not carried out, the sections will bleach out after some time.
Verfahren:
The Warthin-Stary stain is a classic silver nitrate stain in which silver nitrate is reduced to metallic silver with the aid of a hydroquinone-containing developer. The reaction is stopped after a certain time by a rinsing step. As a result, the tissue is stained yellow-brown to golden-brown, while bacteria stand out in black. Cell nuclei take on a dark brown colour. The staining is stabilised by a final fixation reaction in sodium thiosulphate. If this step is not carried out, the sections will bleach out after some time.