Carbol-Gentianaviolet solution for GRAM is used for staining bacteria and spermatozoa in medical and histological diagnostics. The main components are phenol and gentianaviolet B, whose synergistic action enables staining. Phenol acts as a mordant that increases the staining capacity, while gentian violet B produces an intense purple stain. Ethanol and distilled aqua serve as solvents to ensure proper dispersion and solubility of the dyes. The final product is a vividly stained specimen that can be easily viewed under a microscope. As an integral part of the GRAM staining kit according to WEIGERT, this solution provides reliable and reproducible results.