Formalin 5 %, phosphate buffer, pH neutral
Formalin 5%, phosphate buffer, pH neutral is recognized as an in vitro diagnostic agent and is widely used in histology and scientific laboratories. It is typically used to fix tissue samples, which is critical for the study of cellular structures and processes. Aqua bidest or ultrapure water, formaldehyde, di-sodium hydrogen phosphate dihydrate and sodium dihydrogen phosphate dihydrate together form a solution with chemical stability and adequate pH. Formaldehyde is an aldehyde (HCHO), while the two phosphates, di-sodium hydrogen phosphate dihydrate (Na2HPO4.2H2O) and sodium dihydrogen phosphate dihydrate (NaH2PO4.2H2O), serve as a buffer system to ensure the pH stability of the solution. The reaction between formaldehyde and the biological tissue leads to methylene bridges, which crosslink and stabilize the protein structures within the tissue. This allows observation of the tissue structure without change or disintegration. The molarity of the solution is approximately 1.48 M for formaldehyde, 0.055 M for di-sodium hydrogen phosphate dihydrate, and 0.012 M for sodium di-hydrogen phosphate dihydrate.
Article no.: 19270
fixation of specimen
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Sales restriction: no private users!
UN-Nummer: 0000
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Lagerung: 15 … 25 °C
Haltbarkeit: 12 Monate
product information
Relevant Incredients:
• Formaldehyde ~37%, stabilised
• Sodium di-hydrogen Phosphat 2-hydrate
instruction manual
Instructions / Protocol / Recommendations
Verwendung:
Bei der Fixierung werden alle in der Zelle ablaufenden Prozesse unterbrochen, unter möglichst optimaler Erhaltung des Zustandes und der Struktur des Gewebes. Biochemische Prozesse wie Autolyse, Verwesung und Fäulnis werden wirkungsvoll verhindert. Fixierlösung nach Stieve eignet sich zur Fixierung von Hodengewebe und Eierstockgewebe. Die Fixierlösung ist ausschließlich für die professionelle Anwendung als in-vitro Diagnostikum im histologischen Labor vorgesehen.
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