Staining Kit: TB-Staining with Auramin-Rhodamin
The TB staining kit with auramine-rhodamine is a special set of reagents for microscopic identification of mycobacteria, such as the tuberculosis pathogen. The fluorescent staining method enabled by this kit is particularly sensitive and allows efficient examination of specimens.
The fluorescent dyes auramine and rhodamine bind specifically to the mycolic acids in the cell wall of mycobacteria. The resulting fluorescence allows visualization of the bacteria under UV light, with the mycobacteria exhibiting a reddish-gold fluorescence while the background remains dark and uncolored.
Hydrochloric acid alcohol solution is used to wash out dyes that are not specifically bound, allowing clearer visualization of the bacteria. Potassium permanganate is used as a counterstain to minimize background fluorescence and highlight the specific fluorescence of the mycobacteria.
The recommended process of heat fixation and drying for smears or formalin for sections provides efficient fixation of the material to the glass, which increases the stability of the sample during staining.
The fluorescent dyes auramine and rhodamine bind specifically to the mycolic acids in the cell wall of mycobacteria. The resulting fluorescence allows visualization of the bacteria under UV light, with the mycobacteria exhibiting a reddish-gold fluorescence while the background remains dark and uncolored.
Hydrochloric acid alcohol solution is used to wash out dyes that are not specifically bound, allowing clearer visualization of the bacteria. Potassium permanganate is used as a counterstain to minimize background fluorescence and highlight the specific fluorescence of the mycobacteria.
The recommended process of heat fixation and drying for smears or formalin for sections provides efficient fixation of the material to the glass, which increases the stability of the sample during staining.
Article no.: 15460
Bacteria / sperm staining
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product information
Components of this kit:
• Auramine-Rhodamine Solution, Artikel-Nr.:12907
• Hydrochloric Acid in Ethanol (0.4 % / 70 %), Artikel-Nr.:14179
• Potassium Permanganate 0.5 %, Artikel-Nr.:11152
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