Staining Kit: Safranin - Lightgreen for Cartilages
The kit contains the following components Safranin O solution, which is used to stain proteoglycans and glycosaminoglycans in cartilage tissue. Safranin O binds to acidic polysaccharides and stains them bright red, and light green solution, which serves as a counterstain to visualize collagen fibers and other structures in the extracellular matrix. Light green stains collagen fibers and other cellular structures green.
The combination of Safranin O and light green allows for differential visualization of cartilage structures, staining proteoglycans and glycosaminoglycans red, while collagen fibers and other cellular structures appear green. This staining method is particularly useful for the examination of cartilage tissue and the assessment of articular cartilage in pathological conditions such as osteoarthritis or other degenerative joint diseases. The Safranin Light Green Staining Kit for Cartilage Imaging provides an effective and easy-to-use method for the examination of cartilage tissue in histological specimens.
Article no.: 12287
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product information
Components of this kit:
• Light Green 0.2 % (GOLDNER III), Artikel-Nr.:10267
• WEIGERT stock solution A, Artikel-Nr.:10225A
• WEIGERT stock solution B, Artikel-Nr.:10225B
• Safranin O 0.1 %, aqueous, Artikel-Nr.:12382
Instructions / Protocol / Recommendations
Verwendung:
The Safranin light green staining kit for cartilage imaging has been specially developed for in vitro diagnostics of cartilage tissue. It allows differentiated visualization of cartilage structures and extracellular matrix in tissue samples. This method is particularly useful in the examination of articular cartilage and in the evaluation of pathological conditions such as osteoarthritis or other degenerative joint diseases. The kit contains Safranin O solution and Light Green solution, which are used to stain proteoglycans, glycosaminoglycans and collagen fibers.
Prinzip:
Safranin light green staining is a histological staining method for visualizing cartilage tissue in in vitro diagnostics. In this process, Safranin O selectively binds to acidic cartilage components such as proteoglycans, staining them red. Light Green - Goldner III, on the other hand, stains basic structures such as collagen fibers green. Weigert stock solutions A and B play a role in the pretreatment of tissue samples by staining the cell nuclei blue-black. The combination of these dyes enables differentiated visualization of the various tissue structures and facilitates the identification of cartilage tissue in microscopic specimens.
Verfahren:
Safranin light green staining is a histological staining method for visualizing cartilage tissue in in vitro diagnostics. In this process, Safranin O selectively binds to acidic cartilage components such as proteoglycans, staining them red. Light Green - Goldner III, on the other hand, stains basic structures such as collagen fibers green. Weigert stock solutions A and B play a role in the pretreatment of tissue samples by staining the cell nuclei blue-black. The combination of these dyes enables differentiated visualization of the various tissue structures and facilitates the identification of cartilage tissue in microscopic specimens.
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