Staining Kit: GOMORI’s Trichrome Staining
The special suitability of this staining kit lies in its ability to characterize different tissue components by different colors, enabling a differentiated assessment of tissue composition and structure. It is particularly useful for the analysis of muscle diseases and certain connective tissue disorders.
Chemically, the Gomori trichrome staining kit consists of several components, including Bouin's solution, Weigert stock solutions A and B, Gomori trichrome solution, and Gomori differentiation solution. Each of these solutions contains different reagents that allow specific staining interactions with the components of the tissue.
In terms of how it works, the tissue is first fixed in Bouin's solution, which stabilizes its structure. Then, the Weigert stock solutions are applied to stain the cell nuclei. The Gomori trichrome solution is then used to stain the muscle fibers and collagen structures. Finally, the Gomori differentiation solution allows further fine-tuning of the staining by removing excess color, providing a clearer, differentiated view of the various tissue components.
Article no.: 12046
Staining of tissue samples
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product information
Components of this kit:
• BOUIN Fixing Solution, Artikel-Nr.:10153
• WEIGERT stock solution A, Artikel-Nr.:10225A
• WEIGERT stock solution B, Artikel-Nr.:10225B
• GOMORI’s Trichrome Solution with Light Green, Artikel-Nr.:11974
• GOMORI’s Differentiation Solution, Artikel-Nr.:12050
Instructions / Protocol / Recommendations
Verwendung:
The GOMORI trichrome staining kit is used in histology and pathology for in vitro diagnostics. It allows differential staining and visualization of tissue samples, including connective tissue, muscle fibers, cell nuclei and cytoplasm. George Gomori modified trichrome staining is useful for selective staining and examination of cells and extracellular matrix. The kit uses multiple components, including Weigert hematoxylin solution, trichrome solution, and differentiation solution, for accurate and differentiated viewing of various tissue types.
Prinzip:
The GOMORI trichrome staining kit consists of several solutions used in in vitro diagnostics for the specific staining of tissue or cell structures. The principle of operation is based on the different affinity of the solution components for specific structures. Bouin's solution fixes the tissue, while Weigert stock solutions A and B (hematoxylin components) provide nuclear staining. Gomori trichrome solution stains collagen and muscle fibers in different colors, and Gomori differentiation solution provides additional contrast and highlighting of structural details. This procedure provides detailed and high-contrast results for precise morphological analysis of tissue samples.
Verfahren:
The GOMORI trichrome staining kit consists of several solutions used in in vitro diagnostics for the specific staining of tissue or cell structures. The principle of operation is based on the different affinity of the solution components for specific structures. Bouin's solution fixes the tissue, while Weigert stock solutions A and B (hematoxylin components) provide nuclear staining. Gomori trichrome solution stains collagen and muscle fibers in different colors, and Gomori differentiation solution provides additional contrast and highlighting of structural details. This procedure provides detailed and high-contrast results for precise morphological analysis of tissue samples.
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