EDTA Decalcifying Solution ~ 20 %, pH 7.0 - 7.2
EDTA decalcification solution, with approximately 20% concentration and a pH of 7.0 to 7.2, is used in histology and pathology to prepare bone and calcified tissue specimens for microscopic examination. EDTA (ethylenediaminetetraacetic acid) is a chelating agent that selectively removes calcium ions from tissue, thereby dissolving calcifications.
The 20% EDTA decalcifying solution is prepared by dissolving EDTA in water and then adjusting to a pH of approximately 7.0 to 7.2. The pH of 7.0 - 7.2 is close to the physiological pH, which can help to maintain the morphology and structure of the tissue during the decalcification process. The higher concentration allows accelerated decalcification of tissue specimens by extracting the calcium ions from the tissue without affecting the morphological and cellular structures.
Because the concentration of EDTA solution is higher than that of the 10% or 13% solution, decalcification can be performed in a shorter time. However, this depends on the size and density of the sample and may still take several days. The solution should be changed regularly to ensure efficient decalcification.
The 20% EDTA decalcification solution, with a pH of 7.0 to 7.2, is specifically designed for professional use in histology and pathology laboratories and provides an effective and gentle method for preparing bone and calcified tissue specimens for microscopic examination. The solution is also suitable for decalcification of samples to be prepared for immunohistochemistry or in situ hybridization, as it largely preserves antigenicity and nucleic acids.
The 20% EDTA decalcifying solution is prepared by dissolving EDTA in water and then adjusting to a pH of approximately 7.0 to 7.2. The pH of 7.0 - 7.2 is close to the physiological pH, which can help to maintain the morphology and structure of the tissue during the decalcification process. The higher concentration allows accelerated decalcification of tissue specimens by extracting the calcium ions from the tissue without affecting the morphological and cellular structures.
Because the concentration of EDTA solution is higher than that of the 10% or 13% solution, decalcification can be performed in a shorter time. However, this depends on the size and density of the sample and may still take several days. The solution should be changed regularly to ensure efficient decalcification.
The 20% EDTA decalcification solution, with a pH of 7.0 to 7.2, is specifically designed for professional use in histology and pathology laboratories and provides an effective and gentle method for preparing bone and calcified tissue specimens for microscopic examination. The solution is also suitable for decalcification of samples to be prepared for immunohistochemistry or in situ hybridization, as it largely preserves antigenicity and nucleic acids.
Article no.: 16529
Decalcification of tissue samples
Important Information
UN-Nummer: 0000
Warning labels:
Lagerung: 15 … 25 °C
Haltbarkeit: 12 Monate
product information
Relevant Incredients:
• EDTA
• Sodium hydroxide
Hazard and safety instructions
Signal Word 1: Achtung |
Signal Word 2: exclam, silhouete, , |
Warning labels: |