Barbital buffer is an aqueous solution consisting of a combination of barbital, barbituric acid sodium salt, sodium chloride, magnesium chloride hexahydrate, calcium chloride 2-hydrate and ultrapure water. The solution is filtered using a 0.2 µm top filter to remove particles and impurities.

The main uses of this buffer are in biochemistry and electrophoresis, especially in the separation of proteins and nucleic acids. Due to its ability to maintain the pH and ion concentration in a stable environment, the barbital buffer enables the maintenance of the biological activity of the molecules under investigation.

The chemical operation of the barbital buffer is based on the buffering capacity of barbital and barbituric acid sodium salt, which maintain the pH in the desired range. The presence of sodium chloride, magnesium chloride hexahydrate and calcium chloride 2-hydrate helps stabilize ionic strength and osmotic conditions in the solution, which is critical for conducting electrophoresis experiments.

The special suitability of Barbital Buffer lies in its ability to provide stable conditions in a variety of applications. Its buffering capacity and control of ion concentration make it an excellent choice for the separation and analysis of proteins and nucleic acids in biochemistry and electrophoresis.