BOUIN Fixing Solution
The main use of Bouin fixative solution is in the examination of soft tissues such as testes, ovaries and intestines. It allows excellent preservation of cytoplasmic detail and visualization of extracellular matrix structures such as collagen fibers and reticulin fibers. Fixation with Bouin's solution is particularly advantageous for trichrome staining such as Masson's trichrome staining, as it allows sharp and differentiated visualization of the various tissue components.
The operation of Bouin's fixative solution is based on the chemical reactions of the individual components. Picric acid acts as a fixative that precipitates proteins and preserves cellular details. Formaldehyde stabilizes protein structures and preserves tissue morphology. Acetic acid aids in the fixation of cell nuclei and contributes to the preservation of cell integrity.
The special suitability of Bouin's fixative solution is in the study of tissues where the preservation of cell structures and morphology is important. Examples include reproductive tissues such as ovaries and testes, and inflammatory cells. For optimal fixation results, samples should be properly prepared and incubated in the solution for the appropriate amount of time. After fixation, specimens must be thoroughly washed and further processed to avoid unwanted fixation artifacts and to allow correct histological evaluation.
Article no.: 10153
Fixation of tissue samples
Important Information
Sales restriction: no private users!
UN-Nummer: 0000
Warning labels:
Lagerung: 15 … 25 °C
Haltbarkeit: 24 Monate
product information
Relevant Incredients:
• Picric acid (C.I.: 10305)
• Formaldehyde ~37%, stabilised
• Acetic acid 99%
instruction manual
Instructions / Protocol / Recommendations
Verwendung:
During fixation, all processes taking place in the cell are interrupted, while preserving the condition and structure of the tissue as optimally as possible. Biochemical processes such as autolysis, decay and putrefaction are effectively prevented. Highly hydrous mesenchymal tissues and tissues with fine structures (e.g. testis) can be fixed very well with Bouin's solution. The fixing solution is intended exclusively for professional use as an in vitro diagnostic agent in the histology laboratory.
Prinzip:
Bouin fixative solution is a commonly used fixative in in vitro diagnostics, which due to its components enables effective preservation and hardening of tissue specimens. The solution penetrates the tissue by diffusion and has a protein precipitating effect. In this process, the components bind to tissue or cell structures, stabilizing them and preventing their decomposition or alteration. The acetic acid in the solution helps to keep tissue shrinkage to a minimum while maintaining the stainability of the tissue. This enables subsequent analysis and examination of the fixed samples in in vitro diagnostics.
Verfahren:
Bouin fixative solution is a commonly used fixative in in vitro diagnostics, which due to its components enables effective preservation and hardening of tissue specimens. The solution penetrates the tissue by diffusion and has a protein precipitating effect. In this process, the components bind to tissue or cell structures, stabilizing them and preventing their decomposition or alteration. The acetic acid in the solution helps to keep tissue shrinkage to a minimum while maintaining the stainability of the tissue. This enables subsequent analysis and examination of the fixed samples in in vitro diagnostics.
Hazard and safety instructions
| Signal Word 1: Gefahr |
| Signal Word 2: acid_red, exclam, silhouete, |
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Warning labels: |
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