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BOUIN-HOLLANDE’s Fixative for IHC

Bouin-Hollande Fixing Solution is a modified form of Bouin Fixing Solution specifically optimized for immunohistochemistry (IHC). It provides effective fixation and preservation of tissue samples while preserving morphology and intracellular components without compromising antigenicity.

Bouin-Hollande Fixing Solution is composed of saturated aqueous picric acid, formalin, acetic acid and copper sulfate. The combination of these ingredients provides effective fixation and preservation of cellular and tissue structures while maintaining morphology and intracellular components. The solution also decalcifies and chelates iron and calcium ions, lyses erythrocytes, and enhances IHC visualizations through antigen unmasking.

This fixation solution is widely used in the examination of soft tissues such as testes, ovaries, and intestines because it provides excellent preservation of cytoplasmic details and visualization of extracellular matrix structures, such as collagen fibers and reticulin fibers. Fixation with Bouin-Hollande solution is particularly useful for IHC applications because it allows optimal preservation of antigenic structures required for antibody binding.

In immunohistochemistry, Bouin-Hollande fixation solution is advantageous because it allows gentler fixation that preserves the antigenicity of tissue samples while preserving the structural details and morphology of cells. This is critical for successful IHC analysis, where antibodies are used to detect and localize specific antigens within the tissue.

Article no.: 12588

Fixation of tissue samples

  •   Container size and order number Net price Amount
    BOUIN-HOLLANDE’s Fixative for IHC - 100 ml
    Order number: 12588.00100
    41,51 €
    BOUIN-HOLLANDE’s Fixative for IHC - 250 ml
    Order number: 12588.00250
    53,56 €
    BOUIN-HOLLANDE’s Fixative for IHC - 500 ml
    Order number: 12588.00500
    77,12 €
    BOUIN-HOLLANDE’s Fixative for IHC - 1.000 ml
    Order number: 12588.01000
    148,34 €
    BOUIN-HOLLANDE’s Fixative for IHC - 2.500 ml
    Order number: 12588.02500
    319,66 €

Important Information

UN-Nummer: 0000

Warning labels:

Lagerung: 15 … 25 °C

Haltbarkeit: 6 Monate

safety data sheet


product information

Relevant Incredients:
• Essigsaeure; Kupfer(II)-acetat-hydrat
• Picric acid (C.I.: 10305)
• Formaldehyde ~37%, stabilised
• Acetic acid 99%

Technical informations

specifications

Hazard and safety instructions

Signal Word 1: Gefahr
Signal Word 2: exclam, silhouete, ,
Warning labels: