Ammonium sulfate fixation medium pH 7.25, also known as Michels transport medium, is a special product for the fixation and transport of biological samples. Its main field of application is immunofluorescence diagnostics, especially in skin and mucosal biopsies for the investigation of autoimmune diseases.

The medium is characterized by its ability to preserve antigens in samples while maintaining their structure and reactivity. The chemical mode of operation is based on precipitation of proteins and stabilization of antigen-antibody bonds by ammonium sulfate. Citric acid and magnesium sulfate heptahydrate support fixation, while N-ethylmaleimide acts as a protease inhibitor to prevent protein degradation.

Compared to other fixation media, ammonium sulfate fixation medium pH 7.25 offers the advantage of stable antigen preservation and longer sample storage and transport time. This enables better quality of immunohistochemical assays and allows greater flexibility in scheduling analyses.

Overall, Ammonium Sulfate Fixation Medium pH 7.25 is a valuable solution for immunofluorescence diagnostics, contributing to improved analytical results through its efficient fixation and preservation of antigens and prevention of protein degradation.