Alciangrün 1% solution in 3% acetic acid is a proven histological stain. It is specifically used in the application to identify acidic mucopolysaccharides and collagen fibers in tissues. Its particular suitability lies in its ability to highlight these specific structures by binding with the dye component alciangreen.

The chemical properties of this solution are defined by the combination of aqua bidest, acetic acid and alciangreen. This composition creates an acidic environment, which is necessary to achieve an optimal dyeing effect of the alciangreen.

The reactions during application are characterized by electrostatic interactions between Alciangrün and the acidic groups of the structures to be dyed. The binding of the alciangreen results in a visible green coloration.

The functioning of the solution allows certain cellular components, especially acidic mucopolysaccharides and collagen fibers, to be highlighted by a specific green staining. When pH is graded in conjunction with acridine red and chrysoidin, the solution enables the differentiation of three cell types. Alciangreen stains basophilic substances green, acridine red stains acidic substances red and chrysoidin stains neutrophilic substances yellow.