Alcian blue 1 % (pH 2,5 in acetic acid)
Alcian Blue 1 % (pH 2.5 in acetic acid) is a special staining solution used in histology and cytology. This aqueous solution contains 1% Alcian Blue, a cationic dye that specifically binds to acidic polysaccharides found in various tissues such as mucus, cartilage and other extracellular matrix components. Staining occurs at a pH of 2.5, which increases the dye's selectivity for sulfated glycosamine glycans and acidic mucopolysaccharides.
Alcian blue staining is particularly useful for distinguishing and characterizing mucus structures and for observing changes in mucus production that may be associated with certain disease states. It is also used in combination with other staining methods to provide a differentiated picture of tissue structure.
Alcian blue staining is particularly useful for distinguishing and characterizing mucus structures and for observing changes in mucus production that may be associated with certain disease states. It is also used in combination with other staining methods to provide a differentiated picture of tissue structure.
Article no.: 12696
Detection of mucopolysaccharides
product information
Relevant Ingredients:
• Alcian blue 8GS (C.I.: 74240)
Instructions / Protocol / Recommendations
Verwendung:
Alcianblau wird in der Histologie verwendet, um sauro Mukosubstanzen (saure Mucine und sulfatierte Glykosaminoglykane wie Hyaluronsäure oder Chondroitinsulfat) bei niedrigem pH-Wert (1,0 bis 2,5) selektiv anzufärben. Je nach pH-Wert der Färbelösung können spezifische Mucine nachgewiesen werden. Eine weitere Methode der selektiven Darstellung, ist die Variation der Elektrolytkonzentration. Mit steigender Ionenstärke wird eine Färbung mit Alcianblau verhindert, wodurch eine Differenzierung unterschiedlich stark sulfatierter Mucopolysaccharide sowie Heparine, Heparinsulfate und Keratinsulfate möglich ist.
Hazard and safety instructions
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