Alcian blue 0,5 % (pH 2,5 with acetic acid)
Alcian Blue 0.5% (pH 2.5 with acetic acid) is a staining solution used in histology, medical diagnostics and life sciences. Main applications are visualization and characterization of acidic mucopolysaccharides, proteoglycans and glycosaminoglycans in tissue samples. The special suitability of the solution for staining structures such as cartilage, mucus and extracellular matrix is highlighted.
The chemical properties of Alcian Blue 8GS, a cationic dye, allow selective binding to acidic polysaccharides and glycoproteins. The presence of acetic acid in the solution establishes the optimal pH of 2.5 required for specific binding of the dye to acidic polysaccharides.
The operation of Alcian Blue 0.5% (pH 2.5 with acetic acid) is based on electrostatic interactions between the positively charged dye and the negatively charged acidic polysaccharides. Through this binding, an intense and selective staining of the target structures is achieved.
The chemical properties of Alcian Blue 8GS, a cationic dye, allow selective binding to acidic polysaccharides and glycoproteins. The presence of acetic acid in the solution establishes the optimal pH of 2.5 required for specific binding of the dye to acidic polysaccharides.
The operation of Alcian Blue 0.5% (pH 2.5 with acetic acid) is based on electrostatic interactions between the positively charged dye and the negatively charged acidic polysaccharides. Through this binding, an intense and selective staining of the target structures is achieved.
Article no.: 13587
Detection of mucopolysaccharides
product information
Relevant Ingredients:
• Acetic acid 99%
• Alcian blue 8GS (C.I.: 74240)
Instructions / Protocol / Recommendations
Verwendung:
Alcianblau wird in der Histologie verwendet, um sauro Mukosubstanzen (saure Mucine und sulfatierte Glykosaminoglykane wie Hyaluronsäure oder Chondroitinsulfat) bei niedrigem pH-Wert (1,0 bis 2,5) selektiv anzufärben. Je nach pH-Wert der Färbelösung können spezifische Mucine nachgewiesen werden. Eine weitere Methode der selektiven Darstellung, ist die Variation der Elektrolytkonzentration. Mit steigender Ionenstärke wird eine Färbung mit Alcianblau verhindert, wodurch eine Differenzierung unterschiedlich stark sulfatierter Mucopolysaccharide sowie Heparine, Heparinsulfate und Keratinsulfate möglich ist.