Acetate buffer pH 5.8
The acetate buffer with a pH of 5.8 is widely used in biochemical and biological studies. Its ability to maintain a stable pH makes it particularly suitable for experimental conditions that require precise pH control.
The special suitability of this buffer is that it is used in microbiology and molecular biology, especially in DNA extraction and precipitation. In addition, it can be used as a mobile phase in high performance liquid chromatography (HPLC).
Chemically, the buffer is an aqueous solution of acetic acid and sodium acetate. This pair provides a buffer system that keeps the pH of the solution stable by absorbing excess protons or hydroxide ions that might be generated during chemical reactions.
The acetate buffer works on the principle of chemical equilibrium. In the presence of an acid or base, the acetate ion absorbs or releases protons to stabilize the pH of the solution. Thus, the acetate buffer provides a controlled environment for a variety of biochemical experiments and processes.
The special suitability of this buffer is that it is used in microbiology and molecular biology, especially in DNA extraction and precipitation. In addition, it can be used as a mobile phase in high performance liquid chromatography (HPLC).
Chemically, the buffer is an aqueous solution of acetic acid and sodium acetate. This pair provides a buffer system that keeps the pH of the solution stable by absorbing excess protons or hydroxide ions that might be generated during chemical reactions.
The acetate buffer works on the principle of chemical equilibrium. In the presence of an acid or base, the acetate ion absorbs or releases protons to stabilize the pH of the solution. Thus, the acetate buffer provides a controlled environment for a variety of biochemical experiments and processes.
Article no.: 11173
pH stabilization, DNA extraction, chromatography
product information
Relevant Ingredients:
• Acetic acid 99%
• Sodium acetate tri-hydrate