The o-Toluidine solution is used in clinical chemistry and biomedical research. In particular, it is used to measure glucose and other reducing substances in body fluids. Its particular suitability for these applications is based on its ability to react with glucose and other reducing substances to form a blue color that can be measured spectrophotometrically.

Chemically, o-toluidine solution consists of o-toluidine dissolved in distilled water. o-Toluidine is an aromatic amine compound known for its ability to react with reducing substances.

The operation of the o-Toluidine solution is based on the chemical reaction between o-Toluidine and reducing substances, which produces a colored compound. This color change can be measured quantitatively, allowing the determination of the concentration of reducing substances, such as glucose, in a sample.

In the field of medical diagnostics and life sciences, the o-toluidine solution provides a reliable method for quantifying reducing substances and can thus be considered an important component of various testing procedures.