TRIS / HCl Buffer 0.1 mol/l, pH 7.4
TRIS / HCl buffer 0.1 mol/l, pH 7.4 is a buffer solution widely used in biochemical and molecular biology applications. Its ability to maintain pH close to physiological conditions makes it particularly suitable for applications requiring a stable environment, such as protein extraction and purification.
The chemical properties of this buffer result from the combination of TRIS, an organic amino compound, and hydrochloric acid. TRIS acts as a proton acceptor, while hydrochloric acid acts as a proton donor, resulting in a strong buffering effect in the desired pH range. The interaction of TRIS with the protons of hydrochloric acid keeps the pH stable, minimizing changes in environmental conditions.
This buffer is widely used in histology, medical diagnostics and life science laboratories, where it is used in a variety of experiments, such as Western blot, immunoprecipitation and ELISA.
The chemical properties of this buffer result from the combination of TRIS, an organic amino compound, and hydrochloric acid. TRIS acts as a proton acceptor, while hydrochloric acid acts as a proton donor, resulting in a strong buffering effect in the desired pH range. The interaction of TRIS with the protons of hydrochloric acid keeps the pH stable, minimizing changes in environmental conditions.
This buffer is widely used in histology, medical diagnostics and life science laboratories, where it is used in a variety of experiments, such as Western blot, immunoprecipitation and ELISA.
Article no.: 14070
Preparation of buffer solutions
product information
Relevant Incredients:
• TRIS
• Hydrochloric Acid 1.0 mol/l