TRIS / HCL-Buffer pH 8.0
TRIS / HCL buffer pH 8.0 is mainly used in biochemistry and molecular biology to stabilize biological samples and control enzymatic reactions. TRIS base and hydrochloric acid together form a buffer that effectively maintains pH at 8.0, which is optimal for many biological processes. Sodium azide is added to inhibit the growth of microorganisms to maintain sample purity. In laboratory settings, the TRIS / HCL buffer is shown to be a robust and reliable reagent that helps ensure the accuracy and reproducibility of experimental results. It is an essential reagent for a wide range of experiments, from PCR to protein purification to cell culture.
When calculating the molarity, the concentration of TRIS base is 0.1 M, using hydrochloric acid to adjust the pH. The osmolarity is about 200 mOsm/l. The buffer capacity, based on the pKs value of TRIS at 25°C (8.06), is about 40 mM per pH unit.
When calculating the molarity, the concentration of TRIS base is 0.1 M, using hydrochloric acid to adjust the pH. The osmolarity is about 200 mOsm/l. The buffer capacity, based on the pKs value of TRIS at 25°C (8.06), is about 40 mM per pH unit.
Article no.: 16407
Use as a buffer for stabilizing enzymatic reactions
product information
Relevant Incredients:
• TRIS
• Hydrochloric Acid 37%