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TRIS / HCL-Buffer pH 8.0

TRIS / HCL buffer pH 8.0 is mainly used in biochemistry and molecular biology to stabilize biological samples and control enzymatic reactions. TRIS base and hydrochloric acid together form a buffer that effectively maintains pH at 8.0, which is optimal for many biological processes. Sodium azide is added to inhibit the growth of microorganisms to maintain sample purity. In laboratory settings, the TRIS / HCL buffer is shown to be a robust and reliable reagent that helps ensure the accuracy and reproducibility of experimental results. It is an essential reagent for a wide range of experiments, from PCR to protein purification to cell culture.

When calculating the molarity, the concentration of TRIS base is 0.1 M, using hydrochloric acid to adjust the pH. The osmolarity is about 200 mOsm/l. The buffer capacity, based on the pKs value of TRIS at 25°C (8.06), is about 40 mM per pH unit.

Article no.: 16407

Use as a buffer for stabilizing enzymatic reactions

  •   Container size and order number Net price Amount
    TRIS / HCL-Buffer pH 8.0 - 250 ml
    Order number: 16407.00250
    22,70 €
    TRIS / HCL-Buffer pH 8.0 - 500 ml
    Order number: 16407.00500
    28,62 €
    TRIS / HCL-Buffer pH 8.0 - 1.000 ml
    Order number: 16407.01000
    54,28 €
    TRIS / HCL-Buffer pH 8.0 - 2.500 ml
    Order number: 16407.02500
    104,59 €

Important Information

UN-Nummer: 0000

Lagerung: 15 … 25 °C

Haltbarkeit: 12 Monate

safety data sheet

product information

Relevant Incredients:
• TRIS
• Hydrochloric Acid 37%

Technical informations

specifications

Hazard and safety instructions