TRIS/EDTA-Buffer pH 9,0
The TRIS/EDTA buffer with a pH of 9.0 is an alkaline buffer solution commonly used in molecular biology and biochemistry. The solution consists of two main components: TRIS (tris(hydroxymethyl)aminomethane) and EDTA (ethylenediaminetetraacetic acid).
TRIS is a widely used buffer that maintains stable pH by acting as both an acid (proton donor) and a base (proton acceptor). EDTA, on the other hand, is a chelating agent that binds metal ions, inhibiting the activity of metal-dependent enzymes that could otherwise damage biological samples.
The TRIS/EDTA buffer pH 9.0 is used in various applications, such as DNA and RNA extraction, performing polymerase chain reactions (PCR), and electrophoresis of nucleic acids. In some cases, this buffer is also used for antigen unmasking in immunohistochemistry.
TRIS is a widely used buffer that maintains stable pH by acting as both an acid (proton donor) and a base (proton acceptor). EDTA, on the other hand, is a chelating agent that binds metal ions, inhibiting the activity of metal-dependent enzymes that could otherwise damage biological samples.
The TRIS/EDTA buffer pH 9.0 is used in various applications, such as DNA and RNA extraction, performing polymerase chain reactions (PCR), and electrophoresis of nucleic acids. In some cases, this buffer is also used for antigen unmasking in immunohistochemistry.
Article no.: 12328
Preparation of buffer solutions
product information
Relevant Incredients:
• TRIS
• EDTA