TRIS Buffer pH 7.4 - 7.6
TRIS buffer is a buffer solution with a pH of 7.4 to 7.6 used in many biochemical and molecular biology applications. The name TRIS is derived from the chemical compound tris(hydroxymethyl)aminomethane, which is used as a buffering agent in this solution.
TRIS buffer is widely used in protein and nucleic acid research because it stabilizes the pH of solutions in a range that is optimal for most biological processes. Some applications of TRIS buffer include its use in electrophoresis buffer systems, such as SDS-PAGE (polyacrylamide gel electrophoresis), Western blotting, and agarose gel electrophoresis to separate DNA fragments.
In addition, TRIS buffer is also used in cell culture to stabilize the pH of cell culture media, and in histology and immunohistochemistry to preserve and stain tissue samples. The buffering capacity of TRIS and its low toxicity make it an ideal buffering agent for many biological applications.
TRIS buffer is widely used in protein and nucleic acid research because it stabilizes the pH of solutions in a range that is optimal for most biological processes. Some applications of TRIS buffer include its use in electrophoresis buffer systems, such as SDS-PAGE (polyacrylamide gel electrophoresis), Western blotting, and agarose gel electrophoresis to separate DNA fragments.
In addition, TRIS buffer is also used in cell culture to stabilize the pH of cell culture media, and in histology and immunohistochemistry to preserve and stain tissue samples. The buffering capacity of TRIS and its low toxicity make it an ideal buffering agent for many biological applications.
Article no.: 12023
Preparation of buffer solutions
product information
Relevant Incredients:
• TRIS
• Hydrochloric Acid 1.0 mol/l
• Sodium chloride