TRIS buffer (tris(hydroxymethyl)aminomethane buffer) is an important biological buffer used in a variety of scientific applications to stabilize the pH of solutions. A 0.5 mol/l TRIS buffer with a pH of 7.2 is commonly used in biochemical and cell biology experiments because the slightly basic pH is optimal for many biological reactions.

Some typical applications for TRIS buffer with a pH of 7.2 are:

1. Molecular biology:

TRIS buffer is widely used in the preparation of buffers for agarose gel electrophoresis of nucleic acids and buffers for polyacrylamide gel electrophoresis of proteins.
2. Cell culture: TRIS buffer can be used to adjust the pH of cell culture media.
3. Protein chemistry: TRIS buffer is often used as a component of protein extraction and purification buffers.
4. Enzymatic assays: TRIS buffer is often used as a buffer in enzymatic assays to ensure optimal conditions for enzyme activity.

When using TRIS buffer, it is important to use the correct concentration and pH for the particular experiment or procedure. TRIS buffers can be formulated in a variety of concentrations and pH values to meet the specific requirements of each application.