TRIS buffer 0.02 mol/l pH 8.3 consists of distilled water (H₂O), 2.4228 g TRIS (tris(hydroxymethyl)aminomethane, C₄H₁₁NO₃) and 1 ml hydrochloric acid (HCl). This combination is often used in medical and scientific laboratories, especially in histology to stabilize the pH value when staining tissue samples. Chemically, TRIS reacts with the HCl to create a buffer at pH 8.3 that supports the essential processes during color development by maintaining strict pH conditions. The molarity of the buffer is 0.02 M, which corresponds to its concentration in moles per liter. The addition of hydrochloric acid serves to precisely adjust the pH value. By calculating the osmolarity using the ion multiplier for TRIS and HCl, the osmotic properties, which are important for cellular processes, can be specified. Buffering capacity refers to the ability of the solution to maintain its pH stable when acids or bases are added and is crucial for reproducible results in histological applications. Intended use: Stabilization of pH values.