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Substrate Buffer for Alkaline Phosphatase

Substrate Buffer for Alkaline Phosphatase is widely used in biochemical assays, especially when alkaline phosphatase activity is to be measured. The use of this buffer enables the enzymatic conversion of substrates by alkaline phosphatase, thus facilitating their detection and quantification.

The buffer is specifically designed to create an optimal environment for alkaline phosphatase activity. The magnesium chloride it contains serves as a cofactor for the enzyme, while diethanolamine, as a buffering substance, adjusts the pH to a level optimal for the enzyme.

Chemically, diethanolamine is a weak base and reacts with hydrochloric acid to form a salt that acts as a buffer. The sodium azide serves as a bactericidal agent to prevent microbial contamination of the buffer.

In medical diagnostics and life sciences, this substrate buffer is particularly useful. In immunohistochemistry, for example, alkaline phosphatase is often used as a marker enzyme, and the buffer enables the visualization of this enzyme. In addition, the buffer is used in ELISA procedures to allow the enzymatic conversion of the substrate to enable quantification of the analyte.

Article no.: 13873

Preparation of buffer solutions

  •   Container size and order number Net price Amount
    Substrate Buffer for Alkaline Phosphatase - 100 ml
    Order number: 13873.00100
    13,32 €
    Substrate Buffer for Alkaline Phosphatase - 250 ml
    Order number: 13873.00250
    33,48 €
    Substrate Buffer for Alkaline Phosphatase - 500 ml
    Order number: 13873.00500
    34,04 €
    Substrate Buffer for Alkaline Phosphatase - 1.000 ml
    Order number: 13873.01000
    65,54 €
    Substrate Buffer for Alkaline Phosphatase - 2.500 ml
    Order number: 13873.02500
    137,62 €

Important Information

UN-Nummer: 0000

Warning labels:

Lagerung: 15 … 25 °C

Haltbarkeit: 3 Monate

safety data sheet

product information

Relevant Incredients:
• Sodium azide

• 2-(2-hydroxy-ethylamino)-ethanol
• Hydrochloric Acid 37%

Technical informations

specifications

Hazard and safety instructions

Signal Word 1: Gefahr
Signal Word 2: acid_red, silhouete, ,
Warning labels: