Phosphate Citrate Buffer Solution pH 5.0, is an important component in biochemical and medical research processes. It is frequently used in histology and medical diagnostics as a buffer in biochemical assays. It provides an optimal environment for the function of enzymes and other proteins by maintaining the optimal pH range.

The solution is composed of citric acid and di-sodium hydrogen phosphate, which form a buffer system. This buffer system is able to resist fluctuations in pH and therefore provides a stabilizing environment for biochemical reactions. This stability is also maintained during temperature fluctuations, making the phosphate-citrate buffer solution particularly suitable for processes that require temperature control.

Chemically, the phosphate-citrate buffer solution is a mixed buffer. The citric acid acts as a proton donor and absorbs excess hydroxide ions, while the di-sodium hydrogen phosphate acts as a proton acceptor and binds excess protons. This balance between proton donation and uptake allows the solution to effectively stabilize pH.

The function of the solution relies on this buffering system. By absorbing and releasing protons, the phosphate citrate buffer solution pH 5.0, can keep the pH constant even when acids or bases are added. This maintains the function of biological molecules that are active in a specific pH range.